5-氟尿嘧啶对重症感染大鼠细胞因子的双相调节作用  

Effect of 5-fluorouracil on modulation of immunity-associated cytokine during severe sepsis in rat

作　　者：芦灵军[1] 胥楠[2] 陈晓理[2] 

机构地区：[1]四川省宜宾市第一人民医院普外科,644000 [2]四川大学华西医院普外科,成都610041

出　　处：《中国危重病急救医学》2008年第8期479-481,共3页Chinese Critical Care Medicine

基　　金：四川省宜宾市重点科技计划项目（200603012）

摘　　要：目的观察5-氟尿嘧啶（5-FU）对重症感染大鼠致炎细胞因子和抗炎细胞因子的调节作用，探讨5-FU对重症感染大鼠保护作用的机制。方法将30只健康雌性Wistar大鼠随机分成正常对照组、重症感染组和5-FU治疗组，每组10只。重症感染组以无菌操作技术将大肠杆菌悬液（1．8×10^12 cfu／L）注入大鼠腹腔（10ml／kg），制成重症感染模型；5-FU治疗组在重症感染模型制作成功后40min再经腹腔注射5-FU（50mg／kg）。观察各组大鼠6h死亡率和腹水率，然后处死大鼠，抽取下腔静脉血测定血浆中白细胞介素-6（IL-6）和IL-10的含量，称取肺组织湿重。结果 5-FU治疗组大鼠6h死亡率和腹水率均低于重症感染组（P〈0．05和P〈0．01）；重症感染组大鼠肺湿重较正常对照组增加（P〈0．01），5-FU治疗组大鼠肺湿重较重症感染组降低（P〈0．05），并恢复到正常水平。重症感染组血浆IL-6和IL-10水平明显升高（P均〈0．01）；5-FU治疗组IL-6水平较重症感染组降低（P〈0．01），并恢复到正常水平，IL-10水平较重症感染组降低，但仍高于正常对照组（P均〈0．01）。结论5-FU可减少大鼠血浆致炎细胞因子和抗炎细胞因子的释放，减轻肺水肿，对重症感染具有保护作用。Objective To observe the effect of-5-fluorouracil （5-FU） on modulation of the disorders of proinflammatory and anti-inflammatory cytokines in the severe sepsis as a result of intra-abdominal infection in rat, and to investigate the mechanism of efficacy of 5-FU in the treatment of severe sepsis. Methods Thirty female Wistar rats were randomly divided into three groups： control group, severe sepsis group and 5-FU group. Healthy rats served as control （n = 10）. Severe sepsis was induced by peritoneal injection of 1.8× 10^12 cfu/L E. coli with the volume of 10 ml/kg body weight. In the 5-FU group, 50 mg/kg of 5-FU was injected intraperitonealy 40 minutes after severe sepsis was induced. All rats were sacrificed 6 hours after operation. The mortality and the ascites rate were assessed 6 hours after sepsis. At the same time the wet weight of the lung was measured and venous blood was collected for determination of concentrations of interleukin-6 （IL-6） and IL-10. Results Compared with those of severe sepsis group, the mortality and the ascites rate at 6 hours were decreased significantly in 5-FU group （P〈0.05 and P〈0. 01）. The wet weight of lung in severe sepsis group was higher significantly than that in control group （P〈0. 01）. The lung tissue wet weight in 5-FU group was lower than that of severe sepsis group （P〈0. 05）, but it showed no difference when compared with that of control group. The levels of IL-6 and IL-10 in severe sepsis group were higher significantly than those in control group （both P〈0. 01）. The level of IL-6 in 5-FU group was lower than that in severe sepsis group, but with no difference compared with that of control group. The level of IL-IO in 5-FU group was also lower than that in severe sepsis group, but higher than that in control group （both P〈 0. 01）. Conclusion 5-FU may lower simultaneously the level of proinflammatory IL-6 and anti-inflammatory IL-10, alleviate lung edema and inflammation. 5-FU has the protective effect against severe sepsis

关 键 词：5-氟尿嘧啶 感染 重症 动物模型 细胞因子 

分 类 号：R686[医药卫生—骨科学]