番红花不同药用部位的镇痛和抗炎活性比较研究  

The comparative studies on the analgesic and anti-inflammatory activities of different parts from Crocus sativus

作　　者：朱昱[1] 韩婷[1] 张巧艳[1] 秦路平[1] 

机构地区：[1]第二军医大学药学院生药教研室

出　　处：《药学实践杂志》2008年第4期269-271,共3页Journal of Pharmaceutical Practice

基　　金：上海市自然科学基金(05DZ19729)

摘　　要：目的:扩大名贵药用植物番红花的药用资源,寻找可以替代传统药用部位(柱头)的新药用部位。方法:分别采用醋酸致小鼠扭体和二甲苯致小鼠耳肿胀药理模型,分别以吲哚关辛和地塞米松为镇痛和抗炎药效学实验的阳性对照药,观察番红花球茎、顶芽、侧芽和柱头的乙醇和水提取物的镇痛和抗炎活性。结果:在镇痛实验中,与空白对照组相比,顶芽醇提取物和水提取物、柱头的醇提物和水提物以及侧芽的醇提物在高剂量时(相当于2.0 g生药/kg)都具有显著的镇痛活性(P<0.05或P<0.01);球茎的醇提物和水提物、侧芽醇提取物的低剂量和中剂量以及水提物均未见有镇痛活性;在抗炎试验中,与空白对照组相比,番红花柱头醇提物和侧芽醇提物均显示出显著的抗炎活性(P<0.05或P<0.01)。顶芽醇提物在低剂量(相当于0. 75 g生药/kg)时也具有显著的抗炎活性(P<0.05)。球茎醇提取物和水提物及顶芽、侧芽和柱头的水提物对二甲苯致小鼠耳肿胀没有显著性影响。结论:以番红花的顶芽、侧芽替代传统药用部位柱头,分别用于镇痛和抗炎作用有潜在的应用价值,值得深入研究。Objective： To expand new pharmacological resource, traditional plant Crocus sativus L. and to find new pharmacological active parts to take place of traditional pharmacological part, stigma. Methods： The methods of an abdominal constriction induced by acetic-acid and xylene-induced mice ear edema were used to investigate the analgesic and anti-inflammatory effects of different extracts from Crocus sativus, respectively. Indomethacin and dexamethasone were used as positive control of analgesic and anti-inflammatory tests, respectively. The extracts included EtOH extract of corm （CE）, apical bud （AE）, lateral bud （LE）, stigma （SE）, the aqueous extract of corm （CA）, apical bud （AA）, lateral bud （LA）, and stigma （SA）. Result ：In the analgesic tests ,compared with negative control,the extracts from apical bud and stigma of ethanol and aqueous （AE, SE, AA, SA） and lateral bud extract of ethanol at the dose of 2.0 g/kg showed significant （ P 〈 0.05 or P 〈 0.01 ） analgesic activities. On the contrary, CE, CA, LE（ at the dose of 0.75 g/kg and 1.5 g/kg）, and LA showed no analgesic activities . In the anti-inflammatory tests, SE and LE of Crocus sativus showed significant anti-inflammatory effects compared with negative control. At the dose of 0.75 g/kg, the SE showed significant anti-inflammatory effects. However, CA, AA, LA, SA and CE had no anti-inflammatory activities in the tests of xylene-induced mice ear edema. Conclusion ：The studies of apical bud and lateral bud of Crocus sativus instead of stigma of Crocus sativus on analgesic and anti-inflammation use worth further discussing.

关 键 词：番红花 药用部位 镇痛 抗炎 

分 类 号：R285[医药卫生—中药学]