杏李种间杂交新品种“味王”胚培养与植株再生  

Research on the Embryo Culture and Plantlet Regeneration Of Apricot Plum "Tasteking"

作　　者：李芳东[1] 乌云塔娜[2] 杜红岩[1] 

机构地区：[1]中国林业科学研究院经济林研究开发中心,河南郑州450003 [2]中南林业科技大学经济林育种与栽培国家林业局重点实验室,湖南长沙410004

出　　处：《中南林业科技大学学报》2008年第3期18-22,共5页Journal of Central South University of Forestry & Technology

基　　金：国家“948”创新项目(2002-C-01)

摘　　要：以杏李品种"味王"幼胚为实验材料,探讨了幼胚愈伤组织诱导、丛生芽分化、生根的最佳实验条件,并成功地培育出了再生植株.结果表明:MS+BA 2.0 mg/L+NAA 0.5 mg/L为愈伤组织诱导的最佳培养基;MS+BA 1.5 mg/L+NAA 0.5 mg/L为丛生芽分化的最适培养基;MS+NAA 0.8 mg/L为最理想的生根培养基;练苗在80%遮阳网下进行,移栽25 d后成活率可达85%.Using tender immature embryos of apricot plum ＂Tasteking＂ as experimental materials, this paper conducted their tissue culture, bud differentiation and rooting under different conditions. The results indicate that MS ＋ BA 2.0 mg/L ＋ NAA 0.5 mg/L is the optimal culture medium for the inducement of undifferentiated tissue; that MS ＋ BA 1.5 mg/L＋ NAA 0.5 mg/L is the best culture medium for the differentiation of crumb-forming buds ; and that MS ＋ NAA 0.8 mg/L is the most ideal culture medium for the rooting. With a hardening of the seedlings under an 80% sunshield network, their survival rate is up to 85% 25 days after transplanting.

关 键 词：生物技术 杏李 “味王” 胚培养 植株再生 

分 类 号：Q813.1[生物学—生物工程] S662.3[农业科学—果树学]