小麦杂交后代的高分子量麦谷蛋白亚基组成分析  

Composition Analysis of High-molecular-weight Subunits of Glutenin of Wheat Hybridization Progenies

作　　者：张素勤[1] 郑竹[1] 李鹏[1] 耿广东[1] 任明见[1] 张庆勤[1] 

机构地区：[1]贵州大学农学院,贵阳550025

出　　处：《种子》2008年第7期23-25,共3页Seed

基　　金：教育部春晖计划(15001D-3);贵州省优秀科技教育人才省长基金(黔省专合字(2007)19);贵州省农业攻关计划(黔科合NY字(2006)3002B;黔科合NY[2008]3017号)

摘　　要：以郑州9023和野二二燕4 F杂交F2代株系为试材,用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)法鉴定分析了杂交后代的高分子量(HMW)麦谷蛋白亚基组成。结果表明,在Glu-A1、Glu-B1和Glu-D1三个位点上分别检测到2、3和1种亚基类型,3位点结合共出现3种组合类型。其中,在Glu-A1位点上出现了1和N亚基,1优质亚基出现的频率高达66.7%;在Glu-B1位点上,13+16亚基出现频率最高(55.6%);Glu-D1位点上出现的全是被世界公认的优质亚基5+10。1、13+16、5+10亚基组合类型出现的频率最高(55.6%),该类型兼有1和5+10优质亚基。The compositions of high-molecular-weight （HMW） subunits of glutenin of the F2 progenies derived from Zhengzhou 9023 × Yeereryan 4 F were researched using sodium dodecyl sulphate-polyacrylamide gel electrophoresis （SDS-PAGE） method in the present study. The results showed that two, three and one HMW subunit types of glutenin were found in the loci of Glu-A1, Glu-B1 and Glu-D1, respectively, and three composition types appeared. For Glu-A1, subunits 1 and N occurred, and the frequency of high quality subunit 1 was as high as 66.7% ;for Glu-B1, the frequency of subunit pair 13 ＋ 16 was the highest （55.6%） ; for Glu-D1, only subunit pair 5 ＋ 10 （regarded as the highest quality subunit pair in the world） appeared. The frequency of composition type 1, 13 ＋ 16 and 5 ＋ 10 was the highest （55.6%）, which contained high quality subunit 1 and subunit pair 5 ＋ 10.

关 键 词：小麦 HMW 谷蛋白亚基 品质育种 

分 类 号：S512.1[农业科学—作物学]