乌司他丁对脂多糖诱导大鼠急性肺损伤作用的研究  

Ulinastatin suppresses inflammatory response in acute lung injury induced by lipopolysacccharide

作　　者：刘虹[1] 安友仲[2] 李芳芳[1] 郭焱[1] 

机构地区：[1]山西医科大学附属第一临床医院ICU,山西太原030001 [2]北京大学医学部人民医院SICU

出　　处：《中国呼吸与危重监护杂志》2008年第4期290-293,I0003,共5页Chinese Journal of Respiratory and Critical Care Medicine

摘　　要：目的探讨蛋白酶抑制剂乌司他丁对内毒素(LPS)诱导的大鼠ALI的保护作用。方法将30只SD雄性大鼠区组随机分为三组:正常对照组、LPS组和UTI组。LPS组经尾经脉注射LPS造成大鼠ALI模型,UTI组在按与LPS组相同标准给LPS的同时给予乌司他丁(UTI)(10万U/kg体重)。4h后取肺组织测量肺湿干重比(W/D),ELISA法检测动脉血及肺组织中白细胞介素-18(IL-18)水平,免疫组化法检测肺组织中核因子κB(NF-κB)的表达,光镜和电镜下观察肺组织病理变化。结果比较肺组织湿干重比、血清及肺组织中IL-18的水平和肺组织NF-κB表达,LPS组高于对照组;UTI组高于对照组但低于LPS组。LPS组肺泡间质充血水肿、炎性细胞浸润明显;UTI组上述现象较轻。LPS组肺巨噬细胞线粒体、内质网肿胀明显;UTI组肺巨噬细胞内质网仅有零散现象,无肿胀。结论乌司他丁可通过降低炎性细胞因子IL-18和NF-κB的表达减轻LPS所致ALI的肺部炎性反应。Objective To investigate the possible role of ulinastatin（UTI） in lipopolysacccharide （LPS）-induced acute lung injury（ALI）. Methods Thirty male SD rats were randomly divided into three groups, ie, a normal control group, a LPS group and a LPS plus UTI group. The rats were injected with 1 mL of normal saline via caudal vein in the control group,with LPS 5 mg/kg via caudal vein in the LPS group, and with UTI 100000 U/kg shortly after injection with LPS in the LPS plus UTI group. The rats were sacrificed 4 h after the injection. Lung wet/dry weight ratio was measured. IL-18 level in serum and lung tissue was determined by ELISA and the expression of NF-kB in lung tissue was determined by immunohistochemistry. Pathological changes of rats＇ lung were observed by optical and electron microscope. Results Compared with the control group, IL-18 level in serum and NF-kB expression in lung tissue were significantly higher in the LPS group（ P 〈 0.01 ）. The IL-8 level was somewhat elevated in the LPS ＋ UTI group but with no significant difference from that in control group was found （ P 〉 0.05 ）. The lung inflammation in the LPS ＋ UTI group was milder than that in the LPS rats. Conclusion UTI can alleviate LPS-induced inflammatory reaction and lung injury in rat model.

关 键 词：急性肺损伤 乌司他丁 白细胞介素18 核因子ΚB 

分 类 号：R563.8[医药卫生—呼吸系统]