热灭活嗜酸乳杆菌黏附于HeLa细胞后肿瘤细胞免疫学性状的变化  

Antitumor effect of heat-killed lactobacilli adhered to cervical carcinoma cells

作　　者：王红艳[1] 吴晓燕[1] 张旭艳[2] 邢永梅[3] 周亚滨[3] 贾继辉[3] 田永杰[4] 

机构地区：[1]潍坊医学院微生物学教研室 [2]潍坊医学院附属医院妇产科,山东潍坊261042 [3]山东大学医学院实验畸形学教育部重点实验室 [4]山东大学附属山东省立医院妇产科,山东济南250012

出　　处：《中国病理生理杂志》2008年第8期1510-1513,共4页Chinese Journal of Pathophysiology

摘　　要：目的:比较热灭活嗜酸乳杆菌黏附于宫颈癌HeLa细胞后对肿瘤细胞免疫学性状的影响。方法:运用流式细胞技术检测热灭活嗜酸乳杆菌黏附于HeLa细胞后免疫分子表达状况,运用MTT法分析淋巴细胞对HeLa细胞的杀伤作用。结果:热灭活的嗜酸乳杆菌黏附于HeLa细胞后不影响MHC-I分子的表达(P>0.05),可显著增强CD80、CD86的表达,并且可以显著增强NK细胞对其杀伤活性(P<0.05)。细胞毒性淋巴细胞(CTL)对热灭活嗜酸乳杆菌黏附的HeLa细胞的杀伤作用显著高于对照组(P<0.05)。结论:热灭活嗜酸乳杆菌黏附于宫颈癌HeLa细胞后可增强其诱导抗肿瘤效应。AIM： To observe the immune property of cervical carcinoma and antitumour effect of heat - killed lactobacilli adhered to HeLa cells. METHODS： Flow cytometry was used to analyze the expression of immune molecules on HeLa cells after adhered with heat - killed lactobacilli. MTT was used to analyze the cytotoxity of lymphocytes on cervical carcinoma cells. RESULTS： Adherence of heat - killed lactobacilli to HeLa cells did not affect the expression of MHC - I molecules （P 〉 0. 05）, but enhanced the expression of CD80, CD86 significantly （P 〈 0. 05 ） in HeLa cells. Adherence of heat - killed lactobacilli to HeLa cells also enhanced the cytotoxity of NK cells. Compared to control, the cytotoxity of CTL to HeLa carcinoma cells was significantly enhanced by the adherence of heat - killed lactobacilli to HeLa cells （ P 〈 0. 05）. CONCLUSION： Heat - killed lactobacilli strengthen the antitumor effect on HeLa cells.

关 键 词：乳杆菌 嗜酸 HELA细胞 免疫 细胞 

分 类 号：R378.992[医药卫生—病原生物学]