鹿茸多肽对大鼠软骨细胞复制性老化的作用  

Role of pilose antler polypeptides on replicative senescence of rat chondrocyte

作　　者：陈晓东[1] 林建华[2] 

机构地区：[1]深圳平乐骨伤科医院,广东深圳518010 [2]福建医科大学附属第一医院

出　　处：《中国骨伤》2008年第7期515-518,共4页China Journal of Orthopaedics and Traumatology

基　　金：福建省科技计划重大资助项目(编号:2004Y018)

摘　　要：目的:对体外传代培养出现复制性老化的大鼠软骨细胞进行鹿茸多肽干预对照实验,观察鹿茸多肽对软骨细胞复制性老化的影响。方法:将第3代软骨细胞分为空白对照组(未加药物)、鹿茸多肽5、10、15μg/ml组传代使之进入第4代,同时以第2代软骨细胞为青年对照组,进行组化检测老化相关β半乳糖苷酶,流式细胞仪分析细胞周期和增殖指数,阿力新蓝染色检测胞外基质硫酸GAG(glycosaminoglycan)含量和结构,RT-PCR(reverese transcript-polymerase chain reaction)检测Ⅱ型胶原、Aggrecan蛋白,对鹿茸多肽抗软骨细胞老化进行分子生物学研究。结果:鹿茸多肽显著抑制老化相关β半乳糖苷酶的表达(P<0.01)、促进鼠软骨细胞增殖、减少G1期细胞含量、促进软骨细胞胞外基质GAG、Ⅱ型胶原、Aggrecan蛋白表达(P<0.01)。结论:鹿茸多肽具有显著的抗软骨细胞复制性老化作用。Objective ：To observe the function of pilose antler polypeptides （PAP） on replicative senescence of rat chondrocyte subeuhivated in vitro by means of PAP intervention and controlled experiment. Methods：The 3rd generation chondrocytes were divided into blank group ,and PAP groups with three different concentration of PAP which were passaged to the 4th generation. Meanwhile,the 2nd generation of cbontrocytes were used as control group. The cbondrocytes in different groups were detected with the method of bistocbemistry for S-A-β-gal,flow cytometry for cell life cycle and proliferation index,alcian blue test for the content and structure of GAG of ECM ,and RT-PCR for type Ⅱ collagen and Aggrecan. Then PAP＇s function was observed regarding the appearance and functional status in the process of cbondrocyte＇s senescence. Results： PAP significantly inhibited chondrocyte＇s express of S-A-β-gal （P〈0.01 ）, promoted chondrocyte＇s proliferation （P〈0.01 ）, reduced cell content on GI phase,enhanced the content of GAG,type Ⅱ collagen and Aggrecan of ECM （P〈0.01）. Conclusion：PAP can significantly resist rat cbondrocyte senescence occurred in subcuhivation.

关 键 词：鹿茸多肽 软骨细胞 细胞衰老 

分 类 号：R392.11[医药卫生—免疫学] R285.5[医药卫生—基础医学]