氧化苦参碱对实验性结肠炎大鼠肠黏膜细胞因子和核因子-κB p65表达的影响  

Effects of Oxymatrine injection on expression of cytokines and nuclear factor-κB p65 in colonic intestinal mucosa of rats with experimental colitis

作　　者：吕建芳[1] 范恒[1] 沈霖[1] 寿折星[1] 庄雄[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院中西医结合科,湖北省武汉市430022

出　　处：《世界华人消化杂志》2008年第20期2289-2294,共6页World Chinese Journal of Digestology

基　　金：中国博士后科学基金资助项目;No.2005037679~~

摘　　要：目的:观察氧化苦参碱注射液对大鼠实验性结肠炎的治疗效果,探讨其作用机制.方法:将40只SD大鼠随机分为4组:正常对照组、模型组、美沙拉嗪组和氧化苦参碱(OMT)组,每组10只.正常对照组未行造模,其余3组大鼠均采用TNBS造模.模型组给予生理盐水肌注,美沙拉嗪组给予美沙拉嗪混悬液灌胃,氧化苦参碱组给予氧化苦参碱注射液肌注.治疗15d后观察大鼠的腹泻、便血症状和结肠病理组织学改变,用ELISA法检测结肠黏膜组织IL-2、IL-10的变化,并用免疫组化技术检测大鼠结肠黏膜核因子(NF)-κBp65的表达.结果:OMT组大鼠腹泻、黏液脓血便症状得到较快控制,大鼠黏膜组织损伤显著改善.与模型组比较,OMT组IL-2减少(102.93±21.10ng/L vs 231.48±40.78ng/L,P<0.05),IL-10增多(50.13±1.40ng/L vs 18.64±0.65ng/L,P<0.05),NF-κB p65显著降低(16.02%±7.27% vs 43.05%±13.80%,P<0.01).与正常组相比,模型组结肠黏膜IL-2升高,IL-10减少,NF-κBp65表达增多,差异均有显著性意义(102.93±21.10ng/L vs 30.44±12.03ng/L,50.13±1.40ng/L vs 58.92±3.70ng/L,16.02%±7.27% vs 9.57%±4.31%,均P<0.01).OMT组与美沙拉嗪组比较,IL-2、IL-10和NF-κB p65的表达无显著性差异.结论:氧化苦参碱注射液治疗大鼠实验性结肠炎有明显效果,其作用机制可能是通过减少IL-2的生成、促进IL-10分泌和抑制NF-κB p65的激活发挥治疗作用.AIM： To evaluate the efficacy and investigate the anti-inflammatory mechanism of Oxymatrine （OMT） injection for the treatment of experimental colitis in rats. METHODS： Forty male rats were randomly assigned to the following groups （n = 10）： normal control, model group, mesalazine group, and OMT treatment group. Acute colitis was induced using TNBS in each group except the normal control. The rats in model group received normal saline in an equal volume as OMT treatment for 15 days. The rats in Mesalazine group were lavaged by Mesalazine for 15 days, and the rats in OMT treatment were intramuscularly injected with OMT for 15 days. The rats in normal control drank water and feed food normally. Diarrhea and bloody stool as well as colonic histology were noted. The levels of IL-2 and IL-10 were determined by ELISA, and nuclear factor （NF）-kB p65 activity was detected using immu- nohistochemistry method. RESULTS： The inflammatory symptoms （diar- rhea, bloody purulent stool） and histological damages of colonic mucosa in OMT treatment group were significantly alleviated. Compared with experimental colitis, the level of IL-2 was significantly decreased （102.93 ± 21.10 ng/L vs 231.48 ± 40.78 ng/L, P 〈 0.05）, the level of IL-10 was significantly increased （50.13 ± 1.40 ng/L vs 18.64 ± 0.65 ng/L, P 〈 0.05） and the expression of NF-KB p65 was significantly reduced （16.02% ± 7.27% vs 43.05% ± 13.80%, P 〈 0.01）. Compared with the normal control, there was elevated expression of NF-kB p65 and IL-2 （16.02% ± 7.27% vs 9.57% ± 4.31%, 102.93 ± 21.10 ng/L vs 30.44 ± 12.03 ng/L, both P 〈 0.01）, and decreased expression of IL-10 （50.13 ± 1.40 ng/L vs 58.92 ± 3.70 ng/L, P 〈 0.01） in colonic mucosa of experimental colitis rats. And the expression of NF-kB p65, IL-2 and IL-10 in the OMT treatment group was similar （no statistically significant difference） to Mesalazine treatment group. CONCLUSION： Oxymatrine injection is effective for experimental colitis in rats. In

关 键 词：结肠炎 氧化苦参碱 细胞因子 核因子-ΚB P65 

分 类 号：R285.5[医药卫生—中药学]