活性污泥中功能性优势未培养微生物的强化与检测研究进展  

Bioaugmentation and testing of the functional uncultureable microorganism in activated sludge

作　　者：周盛[1] 韦朝海[1] 

机构地区：[1]华南理工大学环境科学与工程学院

出　　处：《化工进展》2008年第8期1204-1209,共6页Chemical Industry and Engineering Progress

基　　金：国家高技术研究开发计划“863”计划(2006AA06Z378);国家自然科学基金项目(2006AA06Z378)

摘　　要：阐述功能性未培养微生物强化研究的重要性,介绍了主要包括营养补充法、Biolog法、分子生态学技术的功能性未培养微生物的强化手段,以及主要包括分子生物学技术(DGGE、T-RFLP、RFLP、ARDRA、T-RFLP)、生物标记物法(脂肪酸图谱分析术、醌类图谱分析术)的功能性优势未培养微生物强化后的检测策略。通过这些方法对激活前后的活性污泥中功能性未培养微生物的代谢活性、降解COD能力提高与否进行检测。其目的是优化筛选出能激活功能性优势未培养微生物的激活剂,最终达到提高活性污泥对废(污)水的处理能力。The importance of bioaugmentation research of functional uncultureable microorganism is presented and the methods of bioaugmentation of functional uncultureable microorganism are introduced, such as Biolog technique, molecular ecology technique, and nutrition supplementation. The testing strategy after intensification of the functional uncultureable microorganism is introduced. It includes molecular biology techniques （DGGE, T-RFLP, RFLP, ARDRA, T-RFLP） , biomarker techniques （quinone profile technique, phospholipid fatty acid profile technique） . After the functional uncultureable microorganism is activated, the metabolic activity and the degradation ability of COD are tested by these methods. The aim is to optimize the activator of functional uncultureable microorganism and to improve the ability of activated sludge in wastewater treatment.

关 键 词：生物强化技术 可培养微生物 未培养微生物 激活剂 分子生态学技术 Biolog法 

分 类 号：X701[环境科学与工程—环境工程]