纳米金标记活细胞双光子荧光成像研究  

Two-photon fluorescence imaging of cationic gold nanoparticles labeled fibroblasts and its application

作　　者：李政[1] 张镇西[1] 

机构地区：[1]西安交通大学生命科学与技术学院,生物医学信息工程教育部重点实验室,西安710049

出　　处：《深圳大学学报（理工版）》2008年第3期248-251,共4页Journal of Shenzhen University(Science and Engineering)

基　　金：国家自然科学基金资助项目(60578026);西安交通大学博士论文基金资助项目(DFXJTU2005-06)

摘　　要：阳离子纳米金是纳米金微粒与多聚L赖氨酸的结合物,在生理pH值条件下,标记在原代培养小鼠上皮成纤维细胞的阴离子位点.采用双光子荧光(two-photo fluorescence,TPF)显微和荧光寿命成像(fluorescence lifetime imaging microscopy,FLIM)技术,对阳离子纳米金标记的成纤维细胞进行二维和三维成像,可对小鼠皮肤重建过程中的成纤维细胞进行跟踪.Fibroblasts were primary cultured from nu-nu/mice skin and grown in vitro on a glass surface or inside a dermal regeneration template matrix. 15 nm Cationic gold particles conjugated with poly-L-Lysine were attached on the anionic sites of fibroblasts on the membrane or inside the plasma because of their strong positive charge at physiological pH. Target cells labeled with cationic gold nanoparticles were excited by two-photon laser. Fluorescence and lifetime images were taken by confocal microscope and time correlated single-photon counting （TCSPC） module to verify the fluorescence of the matrix and the luminescence of the gold particles. Here the cationic gold nanoparticles labeled inside the fibroblasts acted as the tracer for tracking cells＇ distribution in skin reconstruction.

关 键 词：阳离子纳米金 双光子荧光成像 荧光寿命成像 皮肤重建 细胞跟踪 

分 类 号：Q362[生物学—遗传学]