沙利度胺对类风湿关节炎T淋巴细胞的免疫调节作用  

The immunoregulatory effects of thalidomide on T-lymphocytes of rheumatoid arthritis patients

作　　者：严利文[1] 李晶[1] 裘影影[1] 尤海燕[2] 焦志军[2] 吴忠[1] 宋晓丽[1] 

机构地区：[1]江苏大学附属医院风湿科,镇江212001 [2]江苏大学附属医院中心实验室,镇江212001

出　　处：《中华风湿病学杂志》2008年第8期524-526,共3页Chinese Journal of Rheumatology

基　　金：江苏省科技厅社会发展项目（BS2005043）;镇江市科技局社会发展项目（SH2004040）;镇江市重点医学人才资助项目（2005009）

摘　　要：目的探讨沙利度胺（Thd）对类风湿关节炎（RA）患者外周血T淋巴细胞的免疫调节作用。方法在植物血凝素（PHA）刺激下，RA患者外周血T淋巴细胞与不同浓度的Thd（2．5、25、100、300、500μg/ml）共同培养。用四甲基噻唑蓝（MTT）比色法检测T淋巴细胞的增殖，用流式细胞术检测T淋巴细胞的凋亡和T淋巴细胞CDl52、CD28的表达情况，用Real—time聚合酶链反应（PCR）检测T淋巴细胞的白细胞介素（IL）-6、IL-10、肿瘤坏死因子（TNF）一QmRNA表达水平。结果在体外，与阴性对照组相比，500μg/ml Thd组显著抑制T淋巴细胞的增殖，促进T淋巴细胞早期凋亡，抑制T淋巴细胞CD3＋CD28＋表达，促进CD8＋CD152＊3K达，而≤300μg/ml，对T淋巴细胞未见明显影响。与对照组相比，（100～500）μg/ml剂量组可抑制T淋巴细胞IL-6、TNF—dmRNA表达，（2．5～500）μg/ml可促进IL-10mRNA的表达。结论Thd可能通过影响RA患者外周血T淋巴细胞增殖、早期凋亡、CD3＋CD28＋和CD8＋CD152＋表达，以及IL-6、IL-10、TNF—dmRNA表达，反转RA患者的免疫平衡失控。Objective To study the immunoregulatory effects of thalidomide on the peripheral blood T-lymphocytes of rheumatoid arthritis patients. Methods MTT was used to detect the effects of different thalidomide concentrations on the proliferation of T-cells. Flow cytometry was used to analyze T-cells early apoptosis and the T-cells subsets in different concentration of thalidomide. The mRNA expression of IL-6,1L- 10 and TNF-α was measured by RT-PCR method. Results The level of thalidomide at 500μg/ml inhibited the proliferation of T-cells and the CD3＋CD28＋ expression of T-cell subsets, but promoted the early apoptosis and the CD8＋CD152＋ expression of T-cell subsets. Thalidomine at any concentration could inhibit the mRNA expression of IL-6,TNF-α. However, the level of thalidomide that could promote the mRNA expression of IL- 10 was 100 μg/ml and 500 μg/ml, Conclusion Thalidomide can inhibit the proliferation of T lymphocytes and the expression of CD3＋CD28＋ on T-cell subsets. It can promote the early apoptosis and the CD8＋CD152＋ expression of T-cell subsets. Thalidomide inhibits the mRNA expression of IL-6 and TNF-α but promote the mRNA expression of IL-10. Thalidomide has immuno-regulatory effects on rheumatoid arthritis T-cells.

关 键 词：沙利度胺 关节炎 类风湿 T淋巴细胞 免疫调节 

分 类 号：R593.22[医药卫生—内科学]