刺五加体细胞胚胎发生过程及解剖学观察  

Anatomic Observation in the Process of Somatic Embryogenesis of Acanthopanax senticosus

作　　者：王义[1] 李仙[2] 赵文君 孙春玉[1] 张美萍[1] 

机构地区：[1]吉林农业大学生命科学学院,长春130118 [2]哈尔滨工业大学(威海)分校,威海264209 [3]吉林省白山市经济开发区管理委员会,白山134300

出　　处：《林业科学》2008年第7期17-22,F0003,共7页Scientia Silvae Sinicae

基　　金：吉林省科技发展计划项目(20051113)

摘　　要：探讨不同外植体、植物生长调节物质种类及配比对刺五加培养物的影响,建立刺五加体胚发生及再生植株体系。结果表明:叶片最易形成愈伤组织,茎次之,根最差,外植体最佳取材时期为4—5月。刺五加愈伤组织最适诱导基质为MS+1.0mg·L-12,4-D+0.5mg·L-16-BA;体细胞胚的诱导、发育和分化的最佳培养基为不添加任何生长调节物质的MS培养基。解剖学观察表明刺五加离体胚胎发生过程与合子胚发育过程相同。This study intended to establish a culture system that would induce somatic embryogenesis of Acanthopanax senticosus. Various plant growth regulators and their ratio were investigated with different explants of A. senticosus. The results showed that the leaf was the best organ to induce callus, followed by the stem, and the root. April and May were the best period to collect explants for the induction. The best induction medium was MS supplemented with 1.0 mg·L-1 2,4-D and 0.5 mg·L-1 6-BA for callus of A. senticosus. MS alone was the best medium for growth and differentiation. The observed anatomy displayed that the ex vivo embryogenesis process was the same as the zygotic embryo development process of A. senticosus.

关 键 词：刺五加 体细胞胚 再生植株 

分 类 号：S718.46[农业科学—林学] Q943.1[生物学—植物学]