偏头痛肝阳上亢型大鼠脾脏淋巴细胞的比较蛋白质组学分析  

Comparative Proteomics Analysis on Splenic Lymphocytes in Migraine Rat Model with Hyperactivity of Liver-yang

作　　者：胡建军[1] 陈泽奇[1] 钟广伟[1] 李炜[1] 尹耀慧[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,湖南长沙410008

出　　处：《中国中医药信息杂志》2008年第8期34-37,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金青年基金(30500644;30472115);湖南省重点学科资助项目(湘教通2001-179)

摘　　要：目的筛选与偏头痛肝阳上亢型相关的蛋白质,进一步揭示偏头痛肝阳上亢证的分子机制。方法用电刺激SD大鼠三叉神经节,并灌服附子汤的方法制造偏头痛肝阳上亢型模型。采用双向凝胶电泳(2-DE)技术分离偏头痛肝阳上亢型大鼠脾脏淋巴细胞的总蛋白,PDQuest7.0图像分析软件识别差异表达的蛋白质点,基质辅助激光解吸电离飞行时间质谱得到相应的肽质量指纹图,搜索数据库鉴定蛋白质。结果鉴定到的10个差异蛋白分别为:氯通道蛋白-1、硫氧还蛋白过氧化物酶-Ⅰ、硫氧还蛋白过氧化物酶-Ⅱ、专利蛋白WO979348序列、Rho GDP解离抑制因子α、肌动蛋白2/3复合物、神经微丝轻肽、蛋白酶体、热休克蛋白-27、膜联蛋白-A1。结论这些蛋白可能与偏头痛肝阳上亢证的形成有关,本实验为进一步揭示偏头痛肝阳上亢证的发病机制提供了新的线索。Objective To explore the molecule mechanisms of migraine with hyperactivity of liver-yang related proteins. Methods Animal model of migraine with hyperactivity of liver-yang was established through electrical trigeminal ganglion stimulation, and by administering orally with Fuzi decoction. The total proteins of splenic lymphocytes in the rats were separated by immobilized pH gradient-based two-dimensional gel electrophoresis. With coomassie blue staining, the 2-DE images were analyzed by PDQuest 7.0 software. Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry （MALDI-TOF-MS） and database were used to identify the differential proteins. Result Ten differential spots protein were identified, and they were chloride intraceUular channel 1, thioredoxin peroxidase I , thioredoxin peroxidase Ⅱ, sequence 7 from patent WO9709348, Rho GDP dissociation inhibitor （GDI） alpha, actin related protein 2/3 complex neurofilament light polypeptide, proteasome subunit beta type and heat shock protein-27, annexin At. Condusion These protein may be involved in the development of migraine with hyperactivity of liver-yang. These data provide useful clues for elucidating the mechanisms of migraine with hyperactivity of liver-yang.

关 键 词：蛋白质组学 偏头痛 肝阳上亢证 脾脏淋巴细胞 大鼠 

分 类 号：R285.5[医药卫生—中药学]