两种X染色质检查方法的比较  

Comparison of the two detection methods in human sex chromatin

作　　者：李桂英[1] 

机构地区：[1]四川省卫生学校,四川成都610100

出　　处：《现代医药卫生》2008年第15期2248-2249,共2页Journal of Modern Medicine & Health

摘　　要：目的:探讨改进检测X染色质的方法。方法:以在校学生的口腔黏膜细胞为材料,分别用石炭酸复红染液直接涂片法和吉姆萨染液扣染法显示X染色质。结果:石炭酸复红染液染色直接涂片法操作简单,用时短,阳性检测率低;吉姆萨染液扣染法操作稍复杂,用时稍长,阳性检出率高,但由于课堂时间限制,中职学生的课堂教学中不易推广。讨论:应用改进的人类性染色质检测方法,可以准确检出性染色质。Objective：To improve the detection method of human sex ：With the oral mucosa cells on the school students as observation materials,the carbolic acid complex direct smear red dye staining method and Giemsa staining dye deduction were used to show X chromatin.Results：The carbolic acid complex direct smear red dye staining method was simple,with short time for operation and a low rate of positive test;while Giemsa staining dye deduction was slightly complicated,with longer time for operation and a higher rate of positive test.However, the students of polytechnic school in the classroom teaching were difficult to be promoted due to the limited time in classroom.Conclusion： Using the improved detection method of human sex chromatin can accurately detect the sex chromatin.

关 键 词：方法 检测 性染色质 比较 

分 类 号：R446[医药卫生—诊断学]