脑梗死肝阳化风证外周血淋巴细胞相关蛋白质鉴定和分析  

Proteromics Analysis and Identification on Peripheral Blood Lymphocyte of Ganyang Huafeng Syndrome of Cerebral Infarction

作　　者：曾年菊[1] 梁清华[1] 熊新贵[1] 区健刚[1] 萧梅芳[1] 张雨星[1] 梁相辉[1] 赵艳[1] 杨波[1] 刘维[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,湖南长沙410008

出　　处：《中国中医药信息杂志》2008年第4期11-15,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金重大研究计划(90409002);湖南省自然科学基金资助(06JJ20012)

摘　　要：目的应用比较蛋白质组学方法分析并鉴定脑梗死肝阳化风证和阴虚生风证的相关蛋白质,探讨中医肝阳化风证及阴虚生风证的本质内涵。方法设计选择脑梗死肝阳化风证、阴虚生风证患者及健康人各15例,提取外周静脉血淋巴细胞总蛋白质,经双向凝胶电泳,考马斯亮蓝染色获取凝胶图谱,PDQuest V7.3.1软件比较分析3组双向凝胶电泳图谱并识别差异表达蛋白质,对有意义的蛋白进行质谱分析和数据库检索鉴定。结果经比较分析,发现肝阳化风证组与阴虚生风证组差异蛋白质点23个,鉴定出22个共15种蛋白质,相同表达蛋白质点8个,鉴定出6个蛋白质点共5种蛋白质。包括代谢酶类、信号传导蛋白、细胞骨架蛋白、抗氧化蛋白等多类蛋白质表达异常。结论脑梗死肝阳化风证与阴虚生风证具有相同蛋白和差异蛋白,提示二者具有相同的物质基础和不同的本质内涵。Objective To analyze and identify the proteins relevant to Ganyang Huafeng Syndrome （GYHF） and Yinxu Shengfeng Syndrome （YXSF） of cerebral infarction, and investigate the essence of GYHF and YXSF. Methods 3 groups, GYHF of cerebral infarction, YXSF of cerebral infarction and healthy people were designed with 15 cases of each group. Total protein were extracted from lymphocyte of peripheral venous blood, and two dimensional electrophoresis was used to separate total proteins from norma land model ratsynovium. Having been stained, proteins were analyzed by PDquest software. Some selected protein spots were identified by peptide mass fingerprint （PMF） based on Matrix assisted laser desorption/ionizationtirne of flight mass spectrometry （MALDI-TOF-MS） and database. Results 23 different protein spots and 8 identical peotein spots were found out from GYHF and YXSF group, 22 proteins were identified from the different protein spots including 15 kinds of proteins and 6 protein spots including 5 kinds of proteins. GYHF of cerebral infarction related with proteins such as metabolic enzymes, signal transmission proteins, cytoskeletal protein, antioxydation proteins and so forth. Conclusion GYHF and YXSF of cerebral infarction have different proteins and identical proteins, which indicates that the two syndromes have the same matter substructure and different essential connotation.

关 键 词：肝阳化风证 脑梗死 质谱分析 蛋白质组学 

分 类 号：R277.733.3[医药卫生—中医学]