麦冬指纹图谱研究  

Comparative Study on Fingerprints of Root of Ophiopogon japonicus

作　　者：陈有根[1] 邬国庆[1] 戴俊东[1] 李慧[2] 

机构地区：[1]北京市药品检验所,北京100035 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国中医药信息杂志》2008年第4期56-58,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：科技部"十五"国家重大科技专项"创新药物和中药现代化"专题(2001BA701A36-2)

摘　　要：目的建立中药麦冬的指纹图谱,为麦冬药材及其制剂的质量控制提供依据。方法采用HPLC梯度洗脱、紫外检测的方法,分别对产于四川绵阳的川麦冬和浙江慈溪的杭麦冬各10批药材进行检测,用国家药典委员会颁布的"中药色谱指纹图谱相似度评价系统A版",计算各药材样品的相似度,并生成麦冬药材对照指纹图谱。结果来源于两个不同产区的药材,产区内样品间相似度多数大于0.95,但产区间样品相互比较,则相似度多数小于0.80,有的竟小于0.50。结论川麦冬和杭麦冬的指纹图谱相差明显,不仅可用于区别两个不同产区的药材,也可用于区别用两个不同产区的药材生产的中成药制剂。Objective To establish the fingerprints for characterization of the chemical components of maldong （root of Ophiopogon japonicus） in two main cultivate regions of China, Sichuan （Chuanmaidong） and Zhejiang （Hangmaidong）. Methods An HPLC-UV analytical methods was applied to detect 70% ethanol extracts of 20 samples from Sichuan and Zhejiang province, a ＂Fingerprint similarity evaluating system for TCM＂ issued by Pharmacopoeia Committee of P. R. China was used to evaluate the similarities all of the samples. Results The fingerprints revealed that the similarities were higher than 0.95 between samples from the same cultivate region, and were lower than 0.80 between samples from different regions of above two. Conclusion The fingerprints of Chuanmaldong and Hangrnaidong were provided with high difference, and the difference can be taken as a most important proof for distinguishing the material medica of Chuanmaidong and Hangmaldong, but also in patent medicine of TCM.

关 键 词：川麦冬 杭麦冬 指纹图谱 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]