HPLC法测定骨痛灵巴布剂中雪上一枝蒿甲素含量  

Determination of Bullatine A in Gutongling Babu Plaster by HPLC

作　　者：李军红[1] 金日显[1] 赵彬 李曼玲[1] 刘淑芝[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]昆明滇虹药业有限公司,云南昆明650106

出　　处：《中国中医药信息杂志》2008年第4期59-60,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立骨痛灵巴布剂中有效成分的含量测定方法。方法采用高效液相色谱法,Kromasil-C18色谱柱,pH 7.3的磷酸盐缓冲溶液-甲醇(28∶72)为流动相,检测波长210 nm,测定骨痛灵巴布剂的有效成分雪上一枝蒿甲素的含量。结果雪上一枝蒿甲素在0.212 0～4.770 0μg范围内线性关系良好(r=0.999 99),平均回收率100.10%,RSD=2.53%。结论该方法精密度高,回收率稳定,重复性好,可以用于骨痛灵巴布剂的质量控制。Objective To establish a method for the determination of BuUatine A in Gutongling Babu plaster. Method The BuUatine A was determined by HPLC on Kromasil-C18 column, and the mobile phase consisted of phosphate buffered solution （pH 7.3） - methanol （28 ： 72）, the detection wavelength was 210 nm. Result A good linear relationship of BuUatine A was showed in the range of 0.2120-4.7700μg （r=0.999 99）, and the average recovery was 100.10%, RSD=2.53% （n=6）. Conclusion The method is accurate and reliable, and can be used for quality control of Gubitong Babu plaster.

关 键 词：骨痛灵 巴布剂 雪上一枝蒿甲素 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]