喹胺醇与鸡血浆蛋白结合的初步研究  

Study on the integratation between Kui'anchun and plasma proteins of chicken

作　　者：高杰[1] 杨志强[2] 李建喜[2] 王学智[2] 兀征 孟嘉仁[2] 张艳[2] 袁晓科[1] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院兰州畜牧与兽药研究所 [3]甘肃省饲料兽药监察所

出　　处：《中兽医医药杂志》2008年第4期5-7,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金：中央级公益性科研院所基本科研业务费专项基金(NCSTE-2002-JKZX-246);甘肃省自然科学基金(3ZS051-A25-064);兰州畜牧与兽药研究所科研基金(MYJJ04-03)

摘　　要：采用平衡透析法结合反相高效液相色谱法(RP-HPLC)测定鸡血浆中喹胺醇的蛋白结合率。色谱条件为ZORBAX Eclipse XDB C18色谱柱(250mm×4.6mm,5μm),流动相为乙腈∶水(40∶60,v/v),流速1.0mL/min,检测波长352nm。结果显示,喹胺醇浓度为1.1、5.5、22.0μg/mL缓冲液中鸡血浆的蛋白结合率分别为84.41%、75.30%、66.96%;透析平衡时间约为30h。表明喹胺醇的鸡血浆蛋白结合率较高,药物浓度与血浆蛋白结合率之间呈反比关系。The binding rate of Kui＇anchun to plasma protein of chicken was determined by using the equilibrium dialysis and reversed- phase high performance liquid chromatography （RP- HPLC）. Conditions of RP- HPLC were ZORBAX Eclipse XDB C18 of chromatographic column （ 250 mm × 4. 6 mm ,5μm） , the mobile phase of aeetonitrile - water （40/60, v/v） , the flow rale of 1.0 ml. · min^ -1 and the UV detecting wavelength of 352 nm. The protein - binding rates were 84.41% ,75.30% and 66.96% al conceutration of 1. 1 μg/mL, 5.5 μg/mL, and 22.0 μg/mL in buffer, respectively. The time of dialysis was about 30 hours. The result showed that the binding rate of Kui＇anchun to plasma protein was high in chicken, while the protein - binding rate was decreased with the increasing of drugs concentration.

关 键 词：喹胺醇 血浆 蛋白结合率 鸡 

分 类 号：S859.7[农业科学—临床兽医学]