重金属镉离子免疫抗原初步制备与鉴定  

Primary preparation and identification of immune antigen chelating heavy metal ion cadmium

作　　者：袁晓科[1] 杨志强[2] 李建喜[2] 王学智[2] 陈化琦[2] 张艳[2] 孟嘉仁[2] 高杰[1] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院兰州畜牧与兽药研究所

出　　处：《中兽医医药杂志》2008年第4期19-21,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金：甘肃省科学技术攻关(2GS064-A41-002-03)

摘　　要：选用螫合剂Isothiocyanobenzyl-EDTA（IEDTA）分别桥接载体蛋白KLH和BSA。络合重金属镉离子，初步制备免疫抗原和检测抗原，免疫Balb／C小鼠并制备抗血清；利用紫外分光光度法、三硝基苯磺酸法（TNBs）和测定抗体效价初步鉴定抗原。建立了重金属镉离子ELISA快速检测技术及单克隆抗体高效特异的免疫与检测抗原。抗原紫外吸收峰与载体蛋白紫外吸收峰有明显差异，2种抗原游离氨基酸含量在33％～40％之间，抗原蛋白含量均高于70％，血清效价在1：81920以上。结果提示，免疫抗原特异性较好。可用于单克隆抗体制备。The immure, antigen and detecting antigen were synthesized with isothiocyanobenzyl -EDTA（ IEDTA ） , carrier prorein KLH or BSA and cadmium nitrate. Batb/C mice were immunized with the antigen to obtain antiserum. Two antigens were identiffed by three methods including ultraviolet speetrophotometry, trinitro- benzene -sulfonic acid （TNBS） and the autiseru m titer. The different ultraviolet absorbance were observed between synthesized antigen and carrier protein, the contents of free amino acid in antigen was from 33% to 40% , the titer of antiserum was above 1：81 920. These results indicated that the immune antigen with obvious specificity was an ideal candidate for monoclonal antibody preparation,

关 键 词：镉 免疫抗原 制备 鉴定 

分 类 号：S852.4[农业科学—基础兽医学]