柱前荧光衍生化HPLC-RIF法测定人血清托吡酯浓度  

Determination the Concentration of Topiramate in Human Serum by HPLC with Pre-column Derivatization

作　　者：曾艳[1] 杨扬[1] 王育琴[1] 沈芊[1] 

机构地区：[1]首都医科大学宣武医院药剂科,北京100053

出　　处：《中国药师》2008年第8期886-889,共4页China Pharmacist

摘　　要：目的:建立血清中托吡酯(topiramate,TPM)浓度的高效液相色谱-荧光测定法。方法:选用金刚烷胺为内标,以二氯甲烷萃取血清中的托吡酯,经氯甲酸芴甲酯(FMOC-Cl)衍生化后用HPLC法对衍生产物进行分析。以pH6乙腈-磷酸盐缓冲液为流动相,流速1 ml·min^(-1)梯度洗脱;荧光检洲器检测波长为315 nm,激发波长为260 nm。结果:色谱图中TPM衍生物与杂质峰分离良好,血清中的内源性杂质无干扰。TPM在0.25～25μg·ml^(-1)浓度范围内有良好的线性关系(r=0.999 1,n= 6),最低检测限为0.25μg·ml^(-1)。日内与日间RSD均<10.0%。结论:本法可以测定人血清中托吡酯浓度。Objective： To establish a RP-HPLC method for determination the concentration of topiramate in human serum. Method： Topiramate was extracted from human serum with dichloromethane and derivatized by reaction with 9-fluorenylmethyl chloroformate （FMOC-C1）. Acetonitrile sodium phosphate buffer （pH = 6） was used as mobile phase and the flow rate was 1 ml.min-1 with a time program to control the ratio of the two contents of the mobile phase;the detecting wavelength was 315 nm and the exicitation wavelength was 260 nm. Amantadine was used as internal standard. Result： The compounds were separated well. The linear range was 0.25 -25 μg·ml-1 （ r = 0. 999 1, n = 6 ） and the detection limit was 0.25 μg·ml-1 （ S/N = 3 ）. The intraday precision and interday precision were both less than 10%. Conclusion： The method is suitable to determine the concentration of topiramate in human serum.

关 键 词：托吡酯 氯甲酸芴甲酯(FMOC-C1) 高效液相色谱法 柱前衍生化 

分 类 号：R97[医药卫生—药品]