HPLC-ELSD法测定心脑贯通滴丸中三七皂苷R_1、人参皂苷Rg_1、人参皂苷Rb_1的含量  

Determiation the Contents of Notoginsenoside R_1,Ginsenoside Rg_1 and Ginsenoside Rb_1 in Xinnao Guantong Dripping Pills by HPLC-ELSD

作　　者：冯向东 高光伟[2] 黄海欣 

机构地区：[1]南阳市药品检验所,河南南阳473000 [2]南阳市中心医院

出　　处：《中国药师》2008年第8期891-893,共3页China Pharmacist

摘　　要：目的:建立心脑贯通滴丸(三七、银杏叶)中三七皂苷R_1、人参皂苷Rg_1、Rb_1的含量测定HPLC-ELSD方法。方法:色谱柱Agilent ODS C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-水,梯度洗脱,ELSD漂移管温度70℃;氮气流速2.0 L·min^(-1);柱温35℃。结果:3种皂苷,线性范围分别为:三七皂苷R_10.26～2.6μg(r=0.999 2),人参皂苷Rg_1 0.8～8.0μg(r= 0.999 8),人参皂苷Rb_1 0.7～7.4μg(r=0.999 0),平均回收率分别为100.6%,102.5%和103.3%;RSD分别为3.3%,3.8%和3.9%(n=5)。结论:结果准确,便于操作,可作为心脑贯通滴丸的质量控制方法之一。Objective： To establish a method for determination the contents of notoginsenoside R1, ginsenoside Rgl and Rb1 in Xinnao Guantong dripping pills by HPLC-ELSD. Method： Agilent ODS C lS column （250 mm× 4.6 mm,5 μm）was used, the acetonitrile-water as mobile phase, gradient elution, the drift tube temperature was 70 ℃. The nitrogen as carrier gas, the flow rate was 2.0 L. min-1 ELSD as detector. Result： The linear ranges of notoginsenoside R1 , ginsenoside Rgl and ginsenoside Rb1 were at 0.26 -2.6μg（r=0.9992）, 0.8 -8.0 μg（r =0.999 8） and 0.7 -7.4 μg（r=0.9990）, the average recoveries（n=5） were 100.6%, 102.5% and 103.3% with RSD 3.3% ,3.8% and 3.9% , respectively. Conclusion： The method is simple and rapid, it can be used for quality control of Xinnao Guantong dripping pills.

关 键 词：高效液相-蒸发光散射检测器 心脑贯通滴丸 三七皂苷R1 人参皂苷Rg1 人参皂苷RB1 

分 类 号：R927.2[医药卫生—药学]