金银花大孔树脂提取物的指纹图谱考察  

Study on HPLC Fingerprints of Macroporous Resin Extracts from Flos Lonicerae Japonicae

作　　者：刘磊[1] 黄鹤 何再安[1] 余子川[1] 刘超英[1] 刘焱文[1] 

机构地区：[1]湖北中医学院中药资源与中药复方省部共建教育部重点实验室,武汉430061 [2]劲牌有限公司

出　　处：《中国药师》2008年第8期918-920,共3页China Pharmacist

摘　　要：目的:建立金银花大孔树脂提取物的HPLC指纹图谱,为金银花大孔树脂提取物的质量控制提供依据。方法:用HPLC方法建立金银花大孔树脂提取物的指纹图谱,Kromasil C_(18)柱(250 mm×4.6 mm,5μm)流动相为:0.4%磷酸-甲醇线性梯度洗脱,检测波长为238 nm。结果:确定了金银花大孔树脂提取物的14批指纹图谱共有模式,进行了相似度比较。结论:方法简便快捷,为金银花大孔树脂提取物的质量控制提供依据。Objective： To establish the HPLC fingerprints of maeroporous resin extracts from Flose lnicerae Japonicae, for provide the Flose Lonicerae Japnnicae extracts of quality control. Method： The HPLC fingerprints of Flose Lonicerae Japonicae were obtained from Agilent 1100 series instrument. The HPLC separation was performed on a Kromasil C18 column（250 mm× 4.6 mm,5μm）gradient eluted with 0.4% phosphoric acid solution-methanol at the flow rate of 1.0 ml-min-1 , the detection wavelength was 238 nm. Result： The mutual mode of HPLC fingerprints was set up, and the similar degrees to the 14 batches of Flose Lonicerae Japonicae extracts were compared. Conclusion： The method is simple and rapid to provide of Flose Lonicerae Japonicae extracts of quality control.

关 键 词：金银花大孔树脂提取物 高效液相色谱法 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]