广藿香醇对β-淀粉样蛋白神经毒性的抑制作用  

Inhibitory Activities of Patchouli Alcohol on Neurotoxicity of β-amyloid Peptide

作　　者：黄晓舞[1] 白林[1] 徐风华[1] 吴永杰[1] 

机构地区：[1]解放军总医院,北京100853

出　　处：《解放军药学学报》2008年第4期338-340,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的研究雌激素受体卢亚型（estrogen receptor β，ERβ）选择性激动剂广藿香醇（patchouli alcohol，PA）对β-淀粉样蛋白（β-amyloidpe ptide，Aβ）片段神经毒性的体外抑制作用。方法Aβ25-35位氨基酸片段（Aβ25-35）作用于人神经母细胞瘤细胞（SH-SY5Y）之前不同浓度PA预防给药，荧光探针法检测细胞内活性氧（reactive oxygen species，ROS）水平和细胞内钙离子浓度（[Ca^2＋]；），流式细胞术检测细胞凋亡率。结果PA（0．05、0．5、5μg·ml^-1）能够抑制Aβ25-35引起的细胞内ROS水平和[Ca^2＋]；升高，使细胞凋亡减少。结论PA对Aβ25-35的神经毒性具有抑制作用。Aim To observe the activities of selective estrogen receptor （ER）β-agonist, patchouli alcohol （PA）, on neurotoxicity of β-amyloid peptide fragment 25 -35 （Aβ 25-35 ）. Methods SH-SYSY cells were pretreated with indicated concentrations of PA prior to β -amyloid peptide fragment 25 -35 （Aβ 25-35 ） exposure. Then the intracellular reactive oxygen species （ROS） level and intracellular calcium concentration （[ Ca^2＋ ]i） were detected using the fluorescence probe, and the apoptosis rate of the cells was detected by flow cytometry. Results Pretreatment with PA （0.05,0.5,5μg·ml^-1） markedly attenuated Aβ 25-35-induced increases of the ROS level and [ Ca^2＋ ]i,resulting in the decrease of the apoptosis rate. Conclusion PA can protect against Aβ 25 -35-induced toxicity.

关 键 词：广藿香醇 Β-淀粉样蛋白 SH-SY5Y细胞 阿尔茨海默病 

分 类 号：R965[医药卫生—药理学]