苯酚-硫酸法测定不同工艺六味地黄口服液中多糖的含量  

Determination of Polysaccharides in Liu Wei Di Huang Oral Liquid(LOL) with Different Techniques Using Phenol-Sulfuric Method

作　　者：韩燕全[1,2] 黄正明[2] 杨新波[3] 刘贺之[4] 曹文斌[4] 吴光夏 

机构地区：[1]中科院生态环境研究中心,北京100085 [2]解放军第302医院药学部,北京100039 [3]解放军总医院老年医学研究所,北京100853 [4]总后勤部卫生部药品仪器检验所,北京100071 [5]不详

出　　处：《解放军药学学报》2008年第4期349-352,共4页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：北京市科委重大项目攻关课题;NoD0205004040521

摘　　要：目的建立六味地黄口服液中多糖的含量测定方法，比较微滤、超滤和醇沉法对多糖含量的影响。方法采用苯酚-硫酸显色法测定六味地黄口服液中多糖的含量。结果样品经苯酚-硫酸显色后于490nm处测定吸收度，在此波长处溶液的吸收度与葡萄糖含量呈良好线性关系。线性范围：12．0—72．0μg·ml^-1，r=0．9998，加样回收率为99．04％，RSD为1．16％（n：6）；3种工艺六味地黄口服液多糖含量以葡萄糖计分别为8．22、7．04、7．24mg·ml^-1。结论苯酚-硫酸显色法操作简便，准确可靠，可作为六味地黄口服液质量控制方法之一，经孔径为0．18μm微滤膜处理后的六味地黄口服液中多糖含量最高。Aim To establish a method for the determination of polysaccharides in LOL and to study the determinations of LOL using microfiltration method, ultrafiltration method and ethanol-sedimentary method. To provide some reference for quality control of LOL. Methods Using Phenol-sulfuric Method to determine the contents of polysaccharides in LOL. Results The absorptions of processed samples were tested at the wavelength of 490nm. The linear range of Glucose was 12.0 -72.0μg·ml^-1, r =0. 999 8;the average recovery was 99.04%, RSD = 1.16% （ n =6）. The contents of polysaccharides of LOL as produced by the three different proccesses were 8.22,7.04 and 7.24mg·ml^ - 1. glucose respectively. Conclusion Phenol-sulfuric acid Method is reliable, and can be used of as a routine method. Contents of polysaccharides in LOL filtrated through microfiltration membrane with a pore size of 0. 18μm were relatively high.

关 键 词：苯酚-硫酸法 六味地黄口服液 多糖 

分 类 号：R927.2[医药卫生—药学]