万寿菊花酶助青贮工艺及其酶活性分析  

Enzymatic silage technology for Tagetes erecta and analysis of enzyme activity in the water-organic solvent coexistence system

作　　者：李大婧[1] 刘春泉[1] 方桂珍[2] 

机构地区：[1]江苏省农业科学院原子能农业利用研究所 [2]东北林业大学材料科学与工程学院,哈尔滨150040

出　　处：《农业工程学报》2008年第7期237-241,共5页Transactions of the Chinese Society of Agricultural Engineering

基　　金：江苏省科技攻关项目(BE2006319);江苏省博士后科研资助计划项目(0602033B)

摘　　要：针对万寿菊花(Tageteserecta)制备叶黄素的传统生产方法中叶黄素损失较多的情况,提出一重酶助青贮处理万寿菊花生产叶黄素的方法。以叶黄素的提取率为指标值,确定青贮过程的工艺参数,在水-有机溶剂共存体系分析了酶的活性。试验结果表明:青贮和烘干过程叶黄素都有损失,在烘干过程损失(16.33%)比青贮过程损失(6.55%10.27%)更多。在青贮阶段添加β-葡聚糖酶或液态纤维素酶30℃青贮5d,可以很好的降解植物细胞壁,利于叶黄素的提取。分别以万寿菊花冻干粉、青贮鲜花为原料,在水-有机溶剂共存体系确定提取过程所需要的最适水量分别为6.8、2.8～3.8mL/g,保证了该体系中较好的酶活性,能够降解植物细胞壁使水解顺利进行。采用对照试验发现在酶-水-有机溶剂共存体系中纤维素酶仍然具有较高活性。Due to lutein loss in traditional production, a biological procedure described for Tagetes erecta in this article combines silage with enzymatic hydrolysis of the plant cell wall. To take lutein extraction rate as an index, the ensilage process parameters were determined, and enzyme activity in the water-organic solvent coexistence system had been analyzed. The results indicated that lutein has a loss in both ensilage and drying process, and lutein in the drying process （16.33%） compared to ensilage process （6.55%, 10.27%） loses more. Adding fl-glucosan enzyme or liquid cellulose in the ensilage stage at 30℃ for 5 d may well degenerate plant cell wall, which is useful for lutein extraction. To take Tagetes erecta frozen dried powder, ensilaged fresh flower as raw materials, the suitable water yield 6.8, 2.8～3.8 mL/g respectively that the extraction process needed in the water-organic solvent coexistence system was determined, which has guaranteed a good enzyme activity, it can degrade the plant cell wall to make hydrolysis carry on smoothly. Through the check experiment we have also found cellulase a higher activity in the enzyme-water-organic solvent coexistence system.

关 键 词：万寿菊花 酶助青贮 叶黄素 提取 酶活性 

分 类 号：Q562[生物学—生物化学] O629.4[理学—有机化学]