高压氧对家兔面神经撞击伤后面神经核内生长相关蛋白变化的影响(论著)  

Effect of hyperbaric oxygen on the change of growth associated protein in facial nucleus after facial nerve contusion in rabbits

作　　者：姜建平[1,2] 姜燕平[1,2] 王国强 刘宝松[1,2] 宫明利 田增兰[1,2] 张金增[1,2] 

机构地区：[1]解放军第254医院 [2]第三军医大学野战外科研究所

出　　处：《中华航海医学杂志》1997年第3期168-171,共4页

摘　　要：目的：研究面神经撞击伤后面神经核内生长相关蛋白（ＧＡＰ－４３）的变化及高压氧（ＨＢＯ）治疗的影响。方法：利用ＢＩＭ－Ⅱ型生物撞击机致家兔右侧面神经干损伤，应用ＳＰ免疫组化染色法观察损伤后１、３、７、１４和２１天（分别用ＨＢＯ治疗及不用ＨＢＯ为对照）面神经核内ＧＡＰ－４３的变化，并用电镜观察面神经干超微结构变化。结果：面神经损伤第１天，伤侧面神经核区ＧＡＰ－４３样免疫反应性神经元（ＧＬＩＮ）开始增多，第１４天其增多达到高峰，第２１天逐渐下降。ＨＢＯ治疗组伤后不同时期ＧＬＩＮ的增多明显高于对照组。伤后第２１天，与对照组相比ＨＢＯ组面神经干电镜观察可见较多的新生髓鞘。结论：ＨＢＯ治疗可使伤后面神经核内ＧＬＩＮ增多，因而有助于面神经损伤后的修复与再生。Aim: To observe the effect of hyperbaric oxygen (HBO) on the change of growth associated protein (GAP 43) in facial nucleus after facial nerve contusion in rabbits. Methods: Facial nerve contusion model was made with BIM Ⅱ biologic impact machine in rabbits. The change of GAP 43 in facial nucleus and ultrastructure of the injured facial nerve at lst, 3rd, 7th, 14 th and 2lst day after the injury either with HBO treatment or without HBO as control groups were observed by means of streptavidin peroxidase(SP) immunohistochemstry and electron microscopy. Results: GAP 43 like immunohistochemic neurons (GLIN) in the facial nucleus of damaged nerve side began to increase at the lst day and reached the peak at the 14th day of injury, and then decreased gradually by the 2lst day. GLIN in HBO group was significantly higher than that of the control at the 14th and 2lst day of injury ( P<0 01 ). There were more new myelinogeny in facial nerve of HBO group under electron microscope on the 21st day of injury when compared with that of the control. Conclusion: HBO may promote injured facial nerve to repair and regenerate by increasing the synthesis of GAP 43.

关 键 词：面神经损伤 高压氧治疗 神经核 生长相关蛋白 

分 类 号：R651.305[医药卫生—外科学] R459.6[医药卫生—临床医学]