传染性支气管炎DN-1分离株S1基因的克隆与序列分析  

Cloning and analysis of S1 gene of Infectious bronchitis virus of DN-1 Strain

作　　者：董秀梅[1] 曾祥伟[1] 张迎疆 魏萍 刘澜澜[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030 [2]陕西省泾阳县畜牧兽医工作站,陕西泾阳713700

出　　处：《黑龙江畜牧兽医》2008年第8期10-12,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：黑龙江省教育厅科学技术研究SARS专项基金项目(2003fz012)

摘　　要：根据已发表的鸡传染性支气管炎病毒(IBV)S1基因序列设计了1对引物,通过RT-PCR特异性扩增出DN-1株的S1基因,产物为1.7kb,与设计相符。同源性比较结果显示,DN-1株与H52、H120株的S1基因核苷酸序列的同源性分别为99.7%、99.2%,表明IBVDN-1分离株与疫苗株的S1基因具有高度的同源性。One pair of primers for amplifying the S1 gene of avian Infectious bronchitis virus （IBV） were designed, with which the predicted 1.7 kb fragment. It was amplified by RT - PCR from IBV DN - 1 strain. The SI gene of DN - 1 strain was sequenced and compared with other strains such as H120, H52 strains, the homology were 99. 71%, 99. 29% respectively. The high homology of the SI gene of IBV DN - 1 strain with those of other IBV vaccine strains had been proved.

关 键 词：传染性支气管炙病毒 DN-1分离株 S1基因 克隆 序列分析 

分 类 号：S852.659.6[农业科学—基础兽医学]