罗格列酮对脂多糖作用下及高糖对大鼠腹膜间皮细胞TLR2mRNA表达的影响  

The Expression of TLR2 mRNA in Rat Peritoneal Mesothelial Cells Stimulated by Lipopolysaccharide,Rosiglitazone and Highglucose

作　　者：李丽燕[1] 马健飞[1] 王力宁[1] 

机构地区：[1]中国医科大学附属第一医院肾内科,沈阳110001

出　　处：《中国医科大学学报》2008年第4期509-512,共4页Journal of China Medical University

摘　　要：目的观察罗格列酮对脂多糖(LPS)作用下大鼠腹膜间皮细胞(RPMC)toll样受体2(TLR2)mRNA表达的影响;观察高糖对RPMC的TLR2mRNA表达的影响。方法胰蛋白酶法行RPMC的原代培养和传代,经鉴定第3代细胞融合至80%时分组。正常对照组:不同浓度LPS(1,10,100μg/ml)作用8h组;10μg/mlLPS作用不同时间(2,6,12h)组;10μg/mlLPS预孵育2h后,加入罗格列酮(10μmol/L)再作用4h组;不同浓度葡萄糖(1.5%,2.5%,4.25%)作用8h组;2.5%葡萄糖作用不同时间(5,10,20h)组。RT-PCR技术检测TLR2mRNA的表达。结果LPS及高糖均可刺激RPMC的TLR2mRNA表达显著增加,呈剂量依赖性(P<0.05),并且LPS作用下RPMC的TLR2mRNA表达增加在12h内呈时间依赖性(P<0.05),高糖作用下RPMC的TLR2mRNA表达增加在20h内呈时间依赖性(P<0.05);罗格列酮可抑制由LPS刺激导致的TLR2mRNA表达上调(P<0.05)。结论LPS及高糖可上调体外培养RPMC的TLR2mRNA表达,罗格列酮可拮抗LPS对TLR2mRNA的表达上调作用。Objective To observe the effect of rosiglitazone on the mRNA expression of toll-like receptor 2 in rat peritoneal mesothelial cell （RPMC） stimulated by Lipopolysaccharide （LPS） and to observe the expression of toll-like receptor 2 in RPMC stimulated by highglucose. Methods RPMC were isolated from rat colic omentum and were incubated with LPS （ 1.0 μg/ml, 10 μg/ml, 100 μg/ml）,or stimulated by Rosiglitazone （10 μmol/L） after incubated with LPS （10 μg/ml） for 2 h. The RPMC were under the stimulation of highglucose （1.5%, 2.5%,4.25%）. RPMC in the control group were just incubated with medium. TLR2 mRNA was detected by RT-PCR. Results Compared with the control group, the expression of TLR2 mRNA was significantly increased in the groups stimulated by LPS and highglucose. In the group pre-incubated with LPS, compared with the group stimulated by LPS, the up-regnlating expression of TLR2 mRNA was significantly inhibited. Conclusion and highglucose can up-regulate the expression of TLR2 mRNA in rat peritoneal mesothelial cell. Rosiglitazone can inhibit the up-regulating effect of LPS on the expression of TLR2 mRNA.

关 键 词：脂多糖 罗格列酮 高糖 腹膜间皮细胞 TOLL样受体2 

分 类 号：R692[医药卫生—泌尿科学]