IGF-1对成兔关节软骨细胞体外增殖的影响  

The Effect of IGF-1 on Proliferation of Articular Chondrocytes of Cultured Adult Rabbit in Vitro

作　　者：赵广[1] 付勤[2] 沈涛[2] 

机构地区：[1]中国医科大学附属第四医院骨科,沈阳110032 [2]中国医科大学 附属盛京医院骨科,沈阳110004

出　　处：《中国医科大学学报》2008年第4期513-514,共2页Journal of China Medical University

摘　　要：目的研究单独应用胰岛素样生长因子1(IGF-1)对成兔的关节软骨细胞体外增殖的促进作用,为成人关节软骨的体外培养扩增的理论提供新思路。方法通过细胞形态学,细胞计数、细胞计量检测成兔关节软骨细胞的存活及增殖。结果成兔关节软骨细胞增殖活跃。结论在IGF-1的单独作用下成兔关节软骨细胞在体外增殖能力明显增强。Objective To study the effect of IGF-1 on proliferation of cultured adult rabbit articular chondrocytes in vitro alone as a new way to provide theoretical basis of culture and proliferation in vitro of adult human articular chondrocytes. Methods The culture and proliferation of adult rabbits articular chondrocytes in vitro were detected by drawing cell growth curve and detecting the survival number of the chondrocytes by MTT test. Results The proliferation of the adult rabbit articular chondrycytes was successful. Conclusion IGF-1 has the effect of promoting cell proliferation on adult rabbit articular chondrocytes cultured in vitro alone.

关 键 词：胰岛素样生长因子1 成年兔 组织工程 种子细胞 

分 类 号：R684.3[医药卫生—骨科学]