采用去β细胞技术建立胰岛α细胞大鼠模型  

Islet α cell rat model established by the β cell-deleting technology

作　　者：潘琳[1] 杜瑞琴[2] 李宏亮[2] 杨文英[2] 李光伟[2] 

机构地区：[1]卫生部中日友好医院临床研究所细胞生物实验室,北京100029 [2]卫生部中日友好医院内分泌科,北京100029

出　　处：《基础医学与临床》2008年第7期760-764,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(30640081)

摘　　要：目的建立一种去除胰岛β细胞的α细胞大鼠模型。方法12周龄SD大鼠分为3组(n=8):正常对照组(NC)、模型1组(M1)和模型2组(M2),M1和M2组大鼠分别腹腔注射1次链脲佐菌素(STZ)100和150 mg/kg,5 d后处死大鼠,胰腺组织匀浆检测胰岛素(Ins)和胰高糖素(Glc)的含量;胰腺组织HE染色,Ins、Glc经免疫组织化学染色并图像定量分析。结果去β细胞大鼠(M1和M2组)胰岛面积约为正常大鼠的1/7,β细胞面积占胰岛面积比例由正常状态的74.3%分别下降到5.4%和5.2%,胰腺匀浆液Ins的含量不到正常的3%,而Glc的含量略有上升。NC组Glc阳性细胞位于胰岛周边,数量较少,M1和M2组Glc阳性的α细胞由周边向中央聚集,α细胞面积占胰岛面积比例由16.4%分别上升到76.5%和74.4%。结论STZ一次大剂量腹腔注射可获得完全去除胰岛β细胞的大鼠模型,且不影响α细胞的形态和功能,建立胰岛α细胞大鼠模型。Objective To establish the islet α cell rat model by the β cell-deleting technology. Methods Tweentyfour normal male SD rats and 12 weeks old were randomly divided into 3 groups, i. e, a normal diet group（ NC, n = 8 ）, the model group 1 （ M1, n = 8 ）, and the model group 2（ M2, n = 8 ）. The rats in M1 and M2 group were injected with 100 mg/kg and 150 mg/kg of streptozocin respectively. Five days later, the rats were sacrificed. The level of insulin（Ins） and Glucagon（Glc） in the pancreatic homogenate was measured. The tail of pancreas were obtained and fixed by Bolins liquid. Immunohistochemistry was performed to evaluate the expression of Ins and Glc in islet cells. Quantitative analysis was executed by image analyzer. Results Compared with the NC group, the total pancreas island areas of beta-cell deleting rats, M1 group and M2 group, are approximately 1/7 of normal control rats. Moreover, the percentages of beta-cell areas from total pancreas island areas decreased from 74. 3% down to 5.4% and 5.2%, respectively. The Ins content in pancreas tissue homogenate of beta-cell deleting rats does not reach 3% of normal ones, While the Glc content unexpectedly increases. Less alpha cells distinguished by Glc positive dying through Immunohistochemistry are observed at periphery of pancreas islands of NC group. With beta cells deletion, the aggregation of alpha cells from periphery to centre of pancreas islands is found in M1 and M2 groups. Furthermore, the percentages of alpha cells area from total pancreas island areas are promoted from 16.4% to 76. 5% ,74.4% ,respectively. Conclusion The islet α cell rat model was established by injecting large dose STZ （100 mg/kg and 150 mg/kg）.

关 键 词：链脲佐菌素 去除胰岛β细胞 α细胞模型 

分 类 号：R587.1[医药卫生—内分泌]