尖锐湿疣患者皮肤损伤中Src同源序列酪氨酸磷酸酶的表达  

Expression level and significance of Src-homology2 domain phosphatase-1 and -2 in condyloma acuminatum

作　　者：陶小华[1] 董玉娥[1] 潘卫利[1] 孟群[1] 程浩[2] 

机构地区：[1]浙江省人民医院皮肤科,杭州 310014 [2]浙江大学附属邵逸夫医院皮肤科

出　　处：《中华传染病杂志》2008年第7期433-435,共3页Chinese Journal of Infectious Diseases

基　　金：浙江省卫生厅资助课题项目（2005A06）

摘　　要：目的探讨尖锐湿疣（CA）患者皮肤损伤中Src同源序列酪氨酸磷酸酶-1（SHP-1）和Src同源序列酪氨酸磷酸酶-2（SHP-2）的不同表达及意义。方法应用原位杂交技术诊断人类乳头瘤病毒（HPV）6／11相关CA．采用SP免疫组织化学染色技术检测40例HPV6／11阳性CA患者皮肤损伤、20例健康对照皮肤（包皮）中SHP-1和SHP-2的表达及分布。计数资料采用x^2检验。结果CA患者皮肤损伤中SHP—1和SHP-2表达阳性细胞主要位于棘细胞层，棕黄着色，阳性反应定位胞质；而健康对照包皮少数阳性着色主要定位于基底层细胞的胞质，呈浅黄着色。CA患者皮肤损伤和健康对照中SHP-1阳性率分别为80％和35％（x^2=11．87，P〈0．01）；SHP-2表达阳性率分别为85％和30％（x^2=18．15，P〈0．01）。CA患者中SHP-1和SHP-2的表达差异无统计学意义（rs=1．0，P〉0．05）。结论CA中SHP-1和SHP2在HPV6／11感染角质形成细胞过度增殖中起调控作用。Objective To investigate the different expression pattern of Src-homology2 domain phosphatase （SHP）-1 and SHP-2 in human papillomavirus （HPV）6/11 infected condyloma acuminatum （CA） and the significance of the difference. Methods HPV6/11 related CA cases were diagnosed by in situ hybridization. The expression and distribution of SHP-1 and SHP-2 were examined by SP immunohistochemistry technique in skin samples from 40 HPV 6/11 positive CA cases and 20 healthy eontrol （ foreskins）. Results The positive rates of SHP-1 and SHP-2 were 80% and 85% respectively in CA, which were significantly higher than those in healthy control cases （only 35% and 30%, respectively, x^2 11. 87, P〈0. 01; x^2=18. 15,P〈0.01） . The SHP-1 and/or SHP-2 positive cells in CA skin lesions were mainly distributed in prickle layer, showing as hrown yellow, with the positive staining located in cytoplasm. Contrastively, the SHP-1 and/or SHP-2 positive cells in healthy controls were rare and mainly distributed in basal layer, showing as pale yellow with the positive staining located in cytoplasm. There was no significant correlation between the expression of SHP-1 and SHP-2 in CA（rs=1.0, P〉0.05）. Conclusion The expressions of SHP-1 and SHP 2 increase in HPV6/11 positive CA, which suggest that with the infection of HPV6-11, SHP-1 and SHP-2 may play a regulatory role in the proliferation of keratinoeytes.

关 键 词：尖锐湿疣 乳头状瘤病毒 人 蛋白质酪氨酸磷酸酶 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]