安络化纤丸联合干扰素γ干预鼠血吸虫性肝纤维化及对肝色素沉积的影响  

The change of the hepatic fibrosis and pigment deposition in mice schistosomai liver fibrosis treated with combination of Anluohuaxian pilule and interferon-γ

作　　者：黄加权[1] 黄海燕[1] 王丹[2] 彭肖潇[2] 黄铁军[1] 艾国[1] 焦云桃[1] 肖非[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院感染科,武汉430030 [2]华中科技大学同济医学院第二临床学院

出　　处：《中华传染病杂志》2008年第7期436-439,共4页Chinese Journal of Infectious Diseases

基　　金：湖北省血吸虫病防治基金资助项目（XFo6c28）

摘　　要：目的评价安络化纤丸联合干扰素γ治疗鼠血吸虫肝纤维化的效应机制及其对肝色素沉积的影响。方法将30只昆明小鼠分为健康对照组、感染对照组及干扰素γ＋安络化纤丸治疗组。采用日本血吸虫尾蚴（40条／只）攻击感染小鼠，建立血吸虫性肝纤维化模型，连续干预8周，观察肝色素沉积及血吸虫卵肉芽肿改变；免疫组织化学检测肝组织Ⅰ型和Ⅲ型胶原的表达；荧光定量法检测肝组织TGF-β1 mRNA、组织病理学评价及电子计算机图像定量分析。对数据进行正态性检验、方差齐性检验及单因素方差分析。结果肝色素沉积百分比与TGF-β1 mRNA量呈相关性，相关系数=0．8；安络化纤丸联合干扰素γ治疗后明显减轻鼠血吸虫肝组织纤维化、减少色素沉着、使虫卵肉芽肿变小、下调Ⅰ及Ⅲ型胶原的表达及减少TGF—β1 mRNA量表达，与感染对照组比较，差异均有统计学意义（P〈0．05）。结论肝色素沉积量与TGF—β1 mRNA量有一定相关性，安络化纤丸联合干扰素γ明显减轻鼠血吸虫性肝纤维化。下调Ⅰ型及Ⅲ型胶原及TGF-β1 mRNA表达、减少色素沉积是其作用机制之一。Objective To evaluate efficacy and mechanism of Anluohuaxian pilule combined with interferon-γ in the treatment of schistosomal liver fibrosis. To preliminarily study on the relationship of pigment deposition in liver and schistosornal liver fibrosis. Methods Thirty Kunming mice were divided into the normal control group, the infection control group and the combination of Anluohuaxian pilule and Interferon-γ treated group. Schistosomal liver fibrosis model was established by infection with 40 Schistosoma japonicum cercariae. The treated group was treated by combination of Anluohuaxian pilule and Interferon-γ for 8 weeks. The changes of pigment deposition and hepatic egg granuloma in Schistosoma japonicum infected mice were observed. Expressions of collagen Ⅰ and Ⅲ were detected by immunohistochemistry. Transforming growth factor （TGF）-β1 was detected by fluorescent polymerase chain reaction（PCR）. Histopathology and computer image analysis were applied to evaluate the change in the liver tissues. Results The amount of pigment deposition in liver was related to the expression of TGF-β1 mRNA （correlation coefficient = 0.8）. Compared to the infection control group, combination of Anluohuaxian pilule and Interferon-γ can lessen hepatic fibrosis （P〈0.05）. The combination therapy can also make pigment deposition less and hepatic granuloma smaller than the infection control group（P〈0.05）. Conclusions Pigment deposition in liver is related to the expression of TGF-β1. Combination of Anluohuaxian pilule and Interferon-γ can lessen hepatic fibrosis in mice infected with Schistosoma japonicurn. It＇s one mechanism to of the comhination therapy down-regulate the expression of collagen Ⅰ ,Ⅲ and TGF β 1.

关 键 词：安络化纤丸 干扰素Ⅱ型 肝硬化 血吸虫病 日本 血色素沉积症 转化生长因子Β 

分 类 号：R532.21[医药卫生—内科学] R575.2[医药卫生—临床医学]