黄瓜幼苗对氯化钠和碳酸氢钠胁迫的生理响应差异  

Differences of physiological responses of cucumber seedlings to NaCl and NaHCO_3 stress

作　　者：张丽平[1] 王秀峰[1] 史庆华[1] 高青海[1] 刘泽洲[1] 

机构地区：[1]山东农业大学园艺科学与工程学院,山东泰安271018

出　　处：《应用生态学报》2008年第8期1854-1859,共6页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(30471187);山东省优秀中青年科学家科研奖励基金项目(2006BS06019);山东农业大学青年科技创新基金资助项目(23407)

摘　　要：采用营养液培养方法,分别用不同浓度(0、25、50和75mmol.L-1)的NaCl和NaH-CO3对黄瓜幼苗进行胁迫处理,研究黄瓜幼苗对NaCl和NaHCO3胁迫的生理响应差异.结果表明:随着胁迫强度的增加,黄瓜植株地上部和地下部生长量、叶片叶绿素含量和相对含水量均呈明显下降趋势,但NaHCO3处理下降幅度大于NaCl处理.随着处理浓度的增加,黄瓜地上部Na+含量显著上升,K+含量显著下降,在相同的Na+浓度下,NaHCO3处理比NaCl处理下降幅度更大,具有更低的K+/Na+.与NaCl处理相比,NaHCO3处理的黄瓜叶片电解质渗漏率、丙二醛、脯氨酸和可溶性糖含量增加幅度更大.NaCl和NaHCO3处理使黄瓜叶片超氧化物歧化酶、抗坏血酸过氧化物酶和脱氢抗坏血酸还原酶活性受到显著诱导,而使过氧化物酶活性受到明显抑制.By a solution culture experiment, cucumber seedlings were treated with different concentration （0, 25, 50 and 75 mmol · L^- 1 ） NaCl and NaHCO3, and their physiological responses were studied. The results showed that with increasing NaCl and NaHCO3 stress, the shoot and root growth, the leaf chlorophyll content and relative water content of the seedlings decreased dramatical- ly, and the decrements were greater under NaHCO3 than under NaCl stress. The shoot Na ^＋ content increased significantly with increasing NaCl and NaHCO3 stress, while shoot K ^＋ content was in adverse. At the same concentration of Na^＋ in culture solution, the addition of NaHCO3 induced a greater decrement of shoot K ^＋ content, and thus, a lower shoot K ^＋/Na^＋ ratio. Under NaHCO3 stress, the leaf electrolyte leakage rate and the leaf MDA, proline, and soluble sugar contents increased much greater, compared with those under NaCI stress. Under NaCl and NaHCO3 stress, the leaf superoxide dismutase （SOD）, ascorbate peroxidase （APX） and dehydroascorbate reductase （DHAR） activities were induced significantly, while the leaf peroxidase （POD） activity was inhibited obviously.

关 键 词：黄瓜 NACL胁迫 NaHC03胁迫 生理响应 

分 类 号：S642.2[农业科学—蔬菜学]