外周血MSCs的分离培养及对G-CSF的动员反应  

The feasibility analysis of MSCs mobilization by G-CSF and its prosthetic effection in traumatic brain injury

作　　者：邓均[1] 邹仲敏[1] 张春雷[2] 王军平[1] 艾国平[1] 粟永萍[1] 

机构地区：[1]第三军医大学军事预防医学院防原医学教研室全军复合伤研究所创伤烧伤与复合伤国家重点实验室,重庆400038 [2]深圳市中医院检验科,广东深圳518033

出　　处：《局解手术学杂志》2008年第4期225-227,共3页Journal of Regional Anatomy and Operative Surgery

基　　金：国家重点基础研究发展规划资助项目("973"项目;2005CB522605);国家自然科学基金资助项目(30200142)

摘　　要：目的建立多种种属外周血间充质干细胞(MSCs)的分离培养方法,探讨其对粒细胞集落刺激因子(G-CSF)的动员反应。方法用percoll(1.131g/mL)分离多种种属外周血单个核细胞,进行贴壁培养MSCs。以G-CSF作为动员剂,培养骨髓和外周血来源的MSCs,计数动员前后的成纤维细胞集落形成单位(CFU-F)。结果对多种种属进行外周血MSCs分离培养,成功率不同。在本实验条件下,可以稳定的直接分离培养出大鼠外周血的MSCs。G-CSF动员后,骨髓来源MSCs的CFU-F数量显著高于对照组(P<0.01);外周血来源MSCs的CFU-F数量也显著高于对照组(P<0.01),接近4倍。结论不同种属外周血MSCs分离培养难易不同,其直接原因是生理条件下外周血中存在的MSCs量少导致的。G-CSF可以有效地动员大鼠骨髓和外周血MSCs。Objective To establish the isolating cultural method of MSCs from some genus peripheral blood （PB） , and explore the mobilization response of MSCs by G-CSF. Methods MSCs from some genus PB were isolated by percoll （ 1. 131 g/mL） and carried out adherent culture. MSCs-derived bone marrow and PB were cultured and its CFU-F were counted after mobilization by G-CSF. Results PB MSCs were isolated and cultureed successfully or unsuccessfully from different genus. Under our experiment condition, rat＇ s PB MSCs could been isolated and cultured steadily. A significantly higher number of CFU-F of BM-derived MSCs and PB-derived MSCs than that of control group after mobilization by G-CSF（ P 〈 0.01 ） ,ahuost 4-fold increase. Conclusion PB MSCs were isolated and cultured successfully or unsuccessfully from different genus, it concerned with a small number of circulating MSCs in BP. We confirmed that G-CSF mobilized BM-derived MSCs, especially PB-derived MSCs by CFU-F assay.

关 键 词：间充质下细胞 动员 粒细胞集落刺激因子 细胞培养 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] Q813.11[医药卫生—基础医学]