RNA干扰技术及应用  

RNA Interference Technique and the Using

作　　者：林盛国[1] 张嵘[1] 王家庆[2] 

机构地区：[1]沈阳药科大学生命科学与生物制药学院,沈阳110016 [2]河北农业大学海洋学院,河北秦皇岛066003

出　　处：《现代检验医学杂志》2008年第4期83-85,共3页Journal of Modern Laboratory Medicine

摘　　要：RNA干扰(RNA interference,RNAi)是由双链RNA(double-stranded RNA,dsRNA)介导的细胞内双链mRNA特异性降解现象,属于转录后的基因沉默机制。RNAi是生物界普遍存在的抵抗病毒入侵、调控基因表达的监控机制。目前已成功用于基因功能和信号转导的研究,在基因治疗方面也显现出良好前景。RNA interference （RNAi） is mediated through small double-stranded RNA molecules （small interfering RNAs, siRNAs） ,and siRNA can induce special mRNA degradation. The special gene is interfered by post-transcriptlonal gene silencing. It is a monitoring mechanism widely found in living nature to defend the virus infection ,and regulate the expression of protein-coding genes. RNAi is now a powerful instrument for functional genomic analyses and signal transduction pathways ,and it will be a potentionally useful method in highly specific dsRNA based gene-silencing therapeutics.

关 键 词：RNA干扰 MRNA 信号传导 基因治疗 

分 类 号：Q756[生物学—分子生物学]