蓖麻毒蛋白的分离纯化及其对南方根结线虫杀灭效果的研究  

Separation and purification of ricin and its killing effect on Meloidogyne incognita

作　　者：金汝城[1] 贾超[1] 杨晓华[1] 谭玉玲[1] 侯楠楠[1] 

机构地区：[1]兰州理工大学生命科学与工程学院,730050

出　　处：《中国医药生物技术》2008年第4期293-296,共4页Chinese Medicinal Biotechnology

摘　　要：目的探讨蓖麻毒蛋白对南方根结线虫的杀灭效果。方法蓖麻子经脱壳后脱脂,冰浴下在磷酸缓冲溶液中搅拌提取2h,用硫酸铵沉淀得到粗蓖麻毒蛋白,测定并计算浓度;通过葡聚糖G-200柱分离纯化蓖麻毒蛋白,测定并计算浓度;采用小叶碟添加法测定杀灭南方根结线虫的效果;并对蓖麻毒蛋白的血细胞凝集活性进行测定。结果分离纯化得到蓖麻毒蛋白的3个组分,组分2的毒性比组分1的毒性强,组分3的杀灭效果不显著。结论蓖麻毒蛋白杀灭南方根结线虫的效果良好,在生物农药方面具备良好的应用前景。Objective To study the killing effect of ricin on Meloidogyne incognita. Methods Ricin seeds were shelled, de-fat, and stired in phosphate buffer solution with ice bath for 2 h for extraction of Ricin. Ammonium sulfate was used to precipite crude ricin protein, and saphdex G-200 column was used to separate and purify ricin. The concentrations of crude ricin protein and purified ricin were calculated respectively. Lobular plate method was applied to determined the killing effect of the ricin on South Meloidogyne incognita. The hemagglutination activity of the ricin was also detected. Results By using Saphdex G-200 column, we found three fractions in the ricin. Fraction 2 is stronger than fractionl, and the killing rate of fraction 3 is low. Conclusion Ricin can kill the the South Meloidogyne incognita, indicating that the substance can be used as a pesticide

关 键 词：毒力 血凝集试验 小叶碟添加法 

分 类 号：S482.51[农业科学—农药学]