绵羊褪黑激素受体1a基因第二外显子的多态性分析  

Polymorphism Analysis for Exon 2 of Melatonin Receptor 1a Gene in Sheep

作　　者：李广录[1] 赵宗胜[1] 阿米娜[1] 许汉峰[1] 薛安勇[1] 

机构地区：[1]石河子大学动物科技学院

出　　处：《石河子大学学报（自然科学版）》2008年第3期303-306,共4页Journal of Shihezi University(Natural Science)

基　　金：科技部国际合作项目(2007DFB30420)

摘　　要：采用2种限制性内切核酸酶MnlⅠ和RsaⅠ对常年发情的湖羊以及季节性发情的中国美利奴羊、罗米丽羊、罗米丽(Romilly Hills)×中国美利奴(新疆军垦型)褪黑激素受体1a(MTNR1A)基因外显子2的824bp扩增产物进行PCR-RFLP分析。结果表明:MTNR1A基因外显子2的在605位碱基处表现出MnlⅠ酶切多态性,在604位碱基处表现出RsaⅠ酶切多态性。χ2适合性检验结果表明4种绵羊MTNR1A基因第二外显子在RsaⅠ和MnlⅠ酶切位点上都达到Hardy-Weinberg平衡状态(P>0.05);4种绵羊在RsaⅠ和MnlⅠ酶切位点上各基因型的分布通过χ2独立性检验,结果表明差异极显著(P<0.01)。The exon 2 of melatonin receptor la （MTNR1A） gene was amplified and a specific fragment of 824bp was obtained in 4 sheep including non-seasonal estrous breeds （Hu sheep） and seasonal estrous breeds （China Merino,Romilly Hills and Romilly Hills x China Merino Sheep）. The 824bp PCR product was digested with restriction endonucleases Mnl Ⅰ and Rsa Ⅰ , and the genetic polymorphism was detected by PCR-RFLP. The results showed that polymorphic Mnl Ⅰ site was detected at base position 605 of the exon 2 of MTNR 1A gene, and polymorphic Rsa Ⅰ site was detected at base nosition 604. At Mnl Ⅰ site and Rsa Ⅰ site of the exon 2 of MTNR 1A gene in 4 sheep breeds, the results of X^2 fitness test indicated that they were all in Hardy-Weinberg equilibrium（ P 〉 0.05）. The results of X^2 independence test indicated that the distribution and constitution of genetypes at Mnl Ⅰ site and Rsa Ⅰ site of the exon 2 of MTNR1A gene showed significant differences among the 4 sheep breeds（ P 〈 0.01 ）.

关 键 词：绵羊 褪黑激素受体 基因 PCR-RFLP 

分 类 号：S826.2[农业科学—畜牧学]