用标准加入法测定不同类型皮革中的禁用偶氮染料  

Application of Standard Addition Methodology for Determination of Banned Azo Dyes in Different Leather Types

作　　者：Lars-Henric Ahlstrom Johan Raab Lennart Mathiasson 龚英[2] 

机构地区：[1]Lund大学分析化学系 [2]四川大学皮革化学与工程教育部重点实验室,四川成都610065

出　　处：《中国皮革》2008年第15期51-55,共5页China Leather

基　　金：欧洲学会提供科研资助(项目编号G6RD-CT-2001-00600620)

摘　　要：将基于微波萃取(MAE)和标准加入法的分析方法用于了牛革、绵羊革和山羊革中,禁用偶氮染料的测定。将溶于甲醇或水的偶氮染料在4个不同水平上应用标准加入法。偶氮染料是通过用连二亚硫酸钠和氯化锡(Ⅱ)还原染料所生成的有害胺类物质的间接方法测得的。对比所得胺类物质的理论值进行准确度评估,同时还对比了未加入染料的革样通过外标校准法所得回收结果。标准加入法所提供的准确度远远高于外标校准法,并且对于大部分胺类物质的回收接近100%。由于使用甲醇或水作溶剂的标准加入法所得结果没有很大区别,因此将染料溶于甲醇这种标准加入法更好,这是因为在加入标准溶液后溶剂(甲醇)的蒸发更快。An analytical procedure based on microwave - assisted extraction （MAE） and standard addition was applied for the determination of banned azo dyes in bovine, sheep, and goat leather. Standard addition at four different concentration levels was performed using azo dyes, dissolved either in methanol or water. All dyes were determined indirectly by measuring their corresponding harmful aromatic amines, formed after reduction by use of sodium dithionite. Comparing found amounts of amines with theoretical target values allowed an assessment of accuracy. The recoveries were also compared with those obtained for non - spiked samples using external standard calibration. The standard addition approaches provide much better accuracy than external standard calibration, with recoveries close to 100% for most amines. Since there is no great difference in recoveries when using methanol or water as solvent, preparation of the dyes in methanol might be preferred because of the faster evaporation of the solvent after spiking

关 键 词：偶氮染料 芳香胺 皮革 微波萃取 标准加入 

分 类 号：TS57[轻工技术与工程—皮革化学与工程]