广州4194份脐带血HLA-A、B、DRB1等位基因型和单倍型频率研究  

Study of the HLA-A, B, DRB1 allele and haplotype frequencies of 4194 cord blood samples in Guangzhou

作　　者：沙文琼[1] 廖灿[1] 李焱[1] 杨昕[1] 汤雪薇[1] 黄以宁[1] 谢杏梅[1] 

机构地区：[1]广州医学院附属广州市妇婴医院广州市脐血库,510180

出　　处：《中华医学遗传学杂志》2008年第4期473-476,共4页Chinese Journal of Medical Genetics

摘　　要：目的对广州脐血库10年来保存的脐血人类白细胞抗原（human leukocyte antigen，HLA）等位基因及单倍型分布特征进行分析。方法采用单克隆板，序列特异引物聚合酶链反应，PCR序列特异性寡核苷酸探针和DNA测序分型方法对广州脐血库内4194份脐带血进行mA-A、B、DRB1等位基因分型。用Arlequin软件计算HLA基因频率和单倍型频率。结果在广州脐血库中，mA．A、B、DRB1等位基因型分别有18，43，13种。累积频率〉50％的显著高频率等位基因是：A＊11，A＊02，A＊24，A＊33，B＊40，B＊15，B＊46，B＊13，DRB1＊12，DRB1＊15，DRB1＊09，DRBl＊04；最常见的单倍型为：A2-B46、B6-DR9、A11-DR12、A2-B6-D1L9。结论广州脐血库脐血捐献者mA．A、B、DRBl等位基因型及单倍型分布具有典型南方人群的特点，此资料有助于为临床移植寻找合适匹配的供受对。Objective To analyze the distribution of the human leukocyte antigen （HLA）-A, B mad DRB1 allele and haplotype in cord blood samples preserved in Guangzhou Cord Blood Bank collected in the last 10 years. Methods The HLA-A, B and DRB1 genotyping of 4194 cord blood samples were detected by Special Monoclonal Tray, PCR-sequence specific promer（PCR-SSP）, PCR-sequence specific oligonucleotide probe（PCR-SSO） and sequence based typing（SBT）. Frequencies of HLA-A, B and DRBI allele and haplotype were calculated by Arlequin software. Results The total numbers of HLA-A, B and DRB1 alleles are 18,43,13 respectively. The obviously high frequency alleles are As ll,As02,A ＊ 24,A ＊ 33,B ＊ 40,B＊ 15,B ＊ 46,B ＊ 13,DRB1 ＊12,DRBI s 15,DRB1 ＊ 09 and DRB1 ＊04, with accumulative frequency of each locus being more than 50%. The most common haplotypes are A2-B46, B46- DR9, All-DR12 and A2-B46-DR9. Conclusion The distribution of HLA-A, B and DRB1 allele and haplotype of cord blood in Guangzhou Cord Blood Bank has typical characteristics of southern Chinese Han population. Authors＇ data may help in searching for appropriate donors.

关 键 词：脐血库 人类白细胞抗原等位基因 单倍型 频率 

分 类 号：R686[医药卫生—骨科学]