甜菜碱对HepG2人肝癌细胞基因组范围DNA甲基化表达的影响  

Effect of betaine on expression of DNA methylation in HepG2 genome

作　　者：季宇彬[1] 高世勇[1] 杨红丹[1] 何立巍[1] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心药物研究所博士后科研工作站,国家教育部抗肿瘤天然药物工程研究中心,黑龙江哈尔滨150076

出　　处：《中草药》2008年第8期1212-1215,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30400352);教育部科学技术研究重点项目(205045);黑龙江省自然科学基金资助项目(D200611);黑龙江省研究生创新基金项目(YJSCX2006-0077HSD)

摘　　要：目的研究甜菜碱对人肝癌细胞HepG2内基因组范围DNA甲基化的影响。方法以浓度0.1、0.2、0.4mol/L甜菜碱作用于人肝癌细胞HepG2 24、48 h后,通过高效毛细管电泳仪(HPCE)检测肿瘤细胞中DNA水解产物中5-甲基-2′-脱氧胞嘧啶(5-methyl-2′-deoxycytidine,5 mdC)水平,来检测甜菜碱对HepG2内基因组范围DNA甲基化表达的影响。结果随着甜菜碱的浓度增大,5 mdC占总体胞嘧啶的峰面积的百分比也相应增大,且有剂量依赖性。结论甜菜碱作为甲基供体,促进HepG2细胞基因组范围DNA甲基化的表达,从而调控细胞周期抑制肿瘤的生长。Objective To study the effect of betaine on the DNA methylation in HepG2 genome. Methods After betaine at concentration of 0. 1, 0. 2, and 0.4 mol/mL was applied to HepG2 cells for 24 and 48 h, the level of 5-methyl-2-deoxycytidine （5 mdC） in the DNA hydrolytic product in the tumor cells was tested using HPCE. Results As the eoneentraion of betaine increased, the percentage of 5 mdC in the peak area of total cytidine also increased in a dosg-dependent manner. Conclusion As a methyl donor, betaine could promote the expression of DNA methylation in the genome of HepG2 cells, thus regulating the cell cycle of cells and inhibiting tumor growth.

关 键 词：甜菜碱 DNA甲基化 高效毛细管电泳仪 

分 类 号：R979.1[医药卫生—药品] R286.91[医药卫生—药学]