菝葜药材脂溶性成分指纹图谱研究  

Fingerprint of hydropholic components in Smilax china

作　　者：周艳林[1,2] 严海[2] 钟小清[2] 王力生[2] 邹节明[1,2] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]桂林三金药业股份有限公司,广西桂林541004

出　　处：《中草药》2008年第8期1246-1248,共3页Chinese Traditional and Herbal Drugs

基　　金：广西优势中草药产业化资助项目(桂科产0444002-1)

摘　　要：目的建立菝葜药材的脂溶性成分的HPLC指纹图谱分析方法。方法采用Phenomenex C18柱(250 mm×4.6 mm,5μm),以甲醇-0.1%的磷酸溶液梯度洗脱,体积流量1.0 mL/min,柱温35℃,检测波长303nm。结果不同产地菝葜药材的指纹图谱有较好的相似度,通过比较对应了山龙眼苷A、落新妇苷、白藜芦醇及黄杞苷4个对照品。结论建立的方法能较好地反映了菝葜中二氢黄酮醇苷及二苯乙烯两类主要成分。Objective To establish a fingerprint of hydropholic components in Smilax china by RPHPLC. Methods The samples were separated on a Phenomenex C18 column with gradient elution of methanol and 0. 1 %phosphoric acid at a flow rate of 1.0 mL/min and detected wavelength at 303 nm. Results The similarity of S. china fingerprints from different habitats is better and four major active constituents in S. china can be detected simultaneously, which named helicioside A, astilbin, resveratrol, and engeletin. Conclusion The fingerprints can be used as a quality control method for dihydroflavonoids and stilbenes in S. china.

关 键 词：菝葜 脂溶性成分 指纹图谱 梯度洗脱 

分 类 号：R282.7[医药卫生—中药学]