老年急性冠脉综合征患者KLOTHO基因C-1818T多态性分析  

KLOTHO Gene C-1818T Polymorphism in Elderly Patients With Acute Coronary Syndrome

作　　者：何国平[1] 金鸣锋[1] 高磊[1] 杨进[2] 王俊宏[2] 沈丽娟[1] 戚传平[3] 杨国英[1] 杨笛[2] 张寄南[2] 

机构地区：[1]江苏大学附属武进医院心内科,江苏省常州市213002 [2]江苏省人民医院心血管病研究所 [3]江苏大学附属武进医院检验科,江苏省常州市213002

出　　处：《中国循环杂志》2008年第3期203-206,共4页Chinese Circulation Journal

基　　金：江苏省高新技术研究(BG2003033)诊断心肌疾病的生物芯片研制与应用

摘　　要：目的:观察中国苏皖地区汉族老年(≥60岁)人群KLOTHO基因第4外显子C-1818T多态性分布,探讨其与老年人急性冠脉综合征(ACS)的可能关系。方法:采用聚合酶链反应及基因芯片技术,检测272例老年ACS患者(ACS组)和139例老年非冠心病者(对照组)的KLOTHO基因C-1818T多态性。结果:(1)与对照组相比:ACS组CT基因型频率明显升高,差异有统计学意义(30.9vs20.9,P=0.031),CC、TT基因型及T等位基因频率差异均无统计学意义(分别为72.7vs65.4、6.5vs3.7和16.9vs19.1,P值均>0.05)。(2)将ACS组按性别进行进一步分析后发现,分别与对照组相比:①男性CT基因型频率明显升高,差异有统计学意义(28.5vs16.7,P=0.041),CC、TT基因型及T等位基因频率差异无统计学意义(分别为75.6vs68.5、7.7vs3.0和16.0vs17.3,P值均>0.05);②女性CC、CT、TT基因型及T等位基因频率差异无统计学意义(分别为68.9vs56.9、26.2vs37.5、4.9vs5.6和18.0vs24.3,P值均>0.05)。在调整相关危险因素并经多元Logistic回归分析后,与对照组相比:ACS组(优势比=1.993,95可信区间=1.180-3.367,P=0.010)和ACS组男性患者(优势比=2.156,95可信区间=1.010-4.602,P=0.047)CT基因型频率均显著升高,差异有统计学意义。结论:在中国苏皖地区汉族老年人群(特别是老年男性)中,ACS患者CT基因型频率升高,提示KLOTHO基因C-1818T多态性CT基因型与老年人特别是老年男性ACS的发病存在相关性。Objective ：To study the distribution of KLOTHO gene C-1818T polymorphism in the Chinese Hart population of Suwan region and to explore the possible associations of the KLOTHO gene C-1818T polymorphism with acute coronary syndrome （ACS-） in the elderly （age≥60 years）. Methods： This study was conducted with a case-control design including 272 patients with ACS （ACS group） and 139 control subjects who were free from coronary artery disease through coherence checks （control group）. The KLOTHO gene C-1818T polymorphism was determined by polymerase chain reaction and gene mieroarray. Results ： （ 1 ） As compaired with the control group, the prevalence of the CT genetype in elderly patients of ACS group was obveriously higher （20. 9% vs 30. 9% ,P =0. 031 ）. But the frequencies of CC,TY genotype and the T allele of the C-1818T in ACS group were not significantly different （72.7% vs 65.4% -6. 5% vs 3.7% -16. 9% vs 19.1%, respectively,P 〉 0. 05）. （2） After subgrouped by sex we found that as eompaired with the control group： （1）The CT genetype in male group of ACS was also obveriously higher （ 16. 7% vs 28.5% ,P =0. 041 ） , the frequencies of CC、TT genotype and the T allele in male group were not significantly different （75.6% vs 68.5% ,7.7% vs 3. 0% ,16.0% vs 17.3% , respectively, P 〉 0. 05 ）. （2）Also the frequencies of CC ,CT、TT genotype and the T allele in male group were not significantly different （68.9% vs 56. 9% 、26.2% vs 37.5 % ,4. 9% vs 5.6% , 18.0% vs 24. 3 % , respectively, P 〉 0. 05 ）. After adjustment for conventional risk factors by muhiple logistic regression analysis, the carrier prevalence of the CT genetype was still differ significantly between elderly （ odds ratio = 1. 993,95% confidence interval was 1. 180 -3. 367 ,P = 0. 010 ） , especially in elderly male patients （odds ratio = 2. 156,95% confidence interval was 1. 010 - 4. 602 ,P = 0. 047 ） with ACS and control subjects. Conclusion： The CT genetype of KLOTHO gene C-1

关 键 词：KLOTHO 基因多态性 急性冠脉综合征 

分 类 号：R541.4[医药卫生—心血管疾病]