李属植物等位酶遗传多样性研究  

Allozymic Genetic Diversity in Genus Prunus

作　　者：颜福花[1] 刘先葆 徐象华 李作洲[4] 郁香荷[5] 吴连海 蔡礼鸿[1] 

机构地区：[1]华中农业大学园艺植物生物学教育部重点实验室,武汉430070 [2]武汉市林业果树科学研究所,武汉430075 [3]浙江省丽水市林业科学研究所,丽水323000 [4]中国科学院武汉植物园,武汉430074 [5]辽宁省果树科学研究所,熊岳115214

出　　处：《华中农业大学学报》2008年第4期515-521,共7页Journal of Huazhong Agricultural University

基　　金：中国科学院知识创新工程重要方向项目(KSCX2-SW-104);武汉市晨光计划项目(20035002016-40)资助

摘　　要：采用双垂直板聚丙烯酰胺凝胶电泳技术,对李属(PrunusL.)植物的6个分类群及其近缘种杏和欧洲稠李共8个分类群进行了等位酶遗传多样性和种间亲缘关系分析。通过对11个酶系统的检测,在16个位点上共检测到72个等位基因,位点最多等位基因数为9,在6个分类群共发现21个稀有基因;李属植物遗传多样性比较丰富,8个分类群的遗传多样性参数中每位点平均等位基因数(A)、多态性位点的比率(P)和平均预期杂合度(He)分别为2.0、57.1%和0.42;李属植物较其它长寿木本植物种间遗传分化水平高(FST=0.156);本研究结果支持欧洲李与黑刺李的亲缘关系比与樱桃李的亲缘关系近的观点。Allozymic genetic diversity of six taxa of Prunus L.,together with its two close species （Padus racemosa and Armeniaca sp. ） was investigated by using vertical slab polyacrylamide gel electro- phoresis. A total of 72 alleles were detected at sixteen loci of the 11 enzyme systems with the largest number of nine at locus Cat-1. Twenty-one rare alleles were found in six taxa sampled in the study. The results revealed that plum accessions had relatively high levels of genetic diversity, with mean number of alleles per locus A = 2.00, percentage of polymorphic loci P = 57. 1%, and mean expected heterozygosity He = 0. 420. The relative magnitude of differentiation among taxa is significantly higher than those of other long lived woody plants （Fsr =0. 156）. The results supported that P. domesitica L. is more genetically close with P. spinosa L. than that with P. cerasifera Ehrh.

关 键 词：李属 等位酶 遗传多样性 

分 类 号：S662.3[农业科学—果树学]