尖吻蝮蛇血凝酶N末端序列测定及其止血活性分析  

N-terminal amino acid sequencing of haemocoagulase from Agkistrodon acutus venom and analysis of its hemostatic activity

作　　者：郑颖[1] 沈居仁[1] 张富强[1] 邱薇[1] 冯子良[1] 范泉水[1] 

机构地区：[1]成都军区疾病预防控制中心军事医学研究所,昆明650032

出　　处：《中国药科大学学报》2008年第4期365-367,共3页Journal of China Pharmaceutical University

摘　　要：目的:测定尖吻蝮蛇血凝酶N末端氨基酸序列,并对其止血活性进行分析。方法:采用Edm an法测定尖吻蝮蛇血凝酶N末端氨基酸序列,采用毛细管电泳估算其等电点,根据纤维蛋白出现时间和全血凝时间研究其止血活性。结果:尖吻蝮蛇血凝酶N末端氨基酸序列为VIGGNECDTNEEHFL,等电点为6.59,使用后能明显缩短纤维蛋白出现时间和全血凝时间。结论:尖吻蝮蛇血凝酶具有较强的止血活性。Aim： To determine the N-terminal amino acid sequence of haemocoagulase from Agkistodon acutus venom and study its homeostatic activity. Methods： The N-terminal amino acid sequence of haemocoagulase was determined using Edman assay. Its isoelectric point was estimated by capillary electrophoresis and its hemostatic activity was analyzed based on the times required for fibrin formation and whole blood coagulation. Results： It was found that the N-terminal amino acid sequence of haemocoagulase was VIGGNECDTNEEHFL and that its isoelectric point was 6. 59. The amino acid sequences of haemocoagulases from Agkistodon acutus venom and Bothrope jararaca venom were proven to be of high homology. It could shortened the times required for fibrin forma- tion and whole blood coagulation obviously. Conclusion： The haemocoagulases from Agkistodon acutus venom possess considerable hemostatic activities.

关 键 词：尖吻蝮蛇 血凝酶 氨基酸序列 止血活性 

分 类 号：R96[医药卫生—药理学]