高效液相色谱法测定核黄素磷酸钠的有关物质  

Determination of related substances in Riboflavin Sodium Phosphate by HPLC

作　　者：郝桂明[1] 唐素芳[1] 

机构地区：[1]天津市药品检验所,天津300070

出　　处：《天津药学》2008年第4期30-31,共2页Tianjin Pharmacy

摘　　要：目的：建立高效液相色谱法测定核黄素磷酸钠有关物质的方法。方法：采用Dikma Diamonsil TMC18（4．6mm×250mm，5μm）色谱柱，甲醇-0．054mol／L磷酸二氢钾溶液（15：85）为流动相，流速1ml／min，检测波长267nm，柱温为40℃。结果：核黄素磷酸钠在8．0～60．0μg／ml浓度范围内呈良好的线性关系（r=0．9998，n=7），精密度试验RSD为0．6％（n=5），最低检出量为2ng。结论：本方法简便、灵敏、准确，能满足核黄素磷酸钠质量控制的要求。OBJECTIVE To establish an HPLC method for determining related substances in riboflavin sodium phosphate. METHODS Chromatographic conditions included Dikma DiamonsilTM C18 （4.6 mm × 150 mm, 5 μm ） column, and the mobile phase was methanol - 0. 054 mol/L KH2PO4 （ 15： 85 ） , the flow rate was 1 ml/min. Detecting wavelength set at 267 nm and the column temperature was 40 ℃. RESULTS A good linearity was obtained from 8.0 to 60.0 μg/ml of riboflavin sodium phosphate （r =0.999 8, n =7）, RSD was 0.6% （ n =5）, The LOQ was 2 ng. CONCLUSION This method is simple, sensitive and accurate. it an be used to control the quality of riboflavin sodium phosphate.

关 键 词：核黄素磷酸钠 高效液相色谱法 有关物质 

分 类 号：R927.11[医药卫生—药学]