RP-HPLC法同时测定畅鼻通颗粒中3种组分的含量  

Simultaneous determination of three components in Changbitong granules by RP-HPLC

作　　者：申涛[1] 徐海燕[2] 姜煜[1] 栾爽[1] 赵怀清[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]沈阳药科大学高等职业技术学院,辽宁沈阳110026

出　　处：《沈阳药科大学学报》2008年第8期642-645,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的建立同时测定畅鼻通颗粒中桂皮酸、黄芩苷和黄芩素含量的反相高效液相色谱法。方法采用反相高效液相色谱法进行含量测定。色谱柱为Diamonsil^TM C18（4．6mm×200mm，5μm）色谱柱，流动相为甲醇-乙腈-体积分数为1％的冰醋酸水溶液（体积比为65：20：115），流速为1．0mL·min^-1，检测波长为280nm。结果桂皮酸在0．82～8．20mg·L^-1内，黄芩苷在31．00～310．00mg·L^-1内，黄芩素在3．20--32．00mg·L^-1内，质量浓度与峰面积线性关系良好，相关系数分别为0．9999、0．9999和0．9997；平均回收率分别为101．1％、100．5％和101．4％；精密度RSD均小于2．0％。结论RP-HPLC法可用于畅鼻通颗粒的质量控制。Objective To develop an RP-HPLC method to determine simultaneously the concentrations of cinnamic acid, baicalin and baicalein in Changbitong （traditiona Chinese medicines） granules. Methods The separation was performed on a DiamonsilTM C18 （4.6 mm × 200 mm, 5 μm） column with a mixture of methanol-acetonitrile-1% acetic acid solution （V： V： V = 65：20：115） as mobile phase. The flow rate was 1.0 mL· min^-1 and the detection wavelength was set at 280nm. Results A good linearity was obtained within the range of 0.82 - 8.20 mg· L^-1 （ r = 0. 999 9） for cinnamic acid, 31.00 - 310.00 mg·L^-1 （ r = 0. 999 9）for baicalin and 3.20- 32.00 mg· L^-1（ r = 0. 999 7） for baicalein. The average recoveries of the three components were 101.1%, 100.5 % and 101.4 %, respectively. The precision was not more than 2.0 %. Conclusions The method is proved to be selective and reproducible; it is suitable for the quality control of Changbitong granules.

关 键 词：畅鼻通颗粒 桂皮酸 黄芩苷 黄芩素 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]