人鼻腔嗅粘膜嗅鞘细胞的原代培养和免疫组化观察  

primary cultures and immunocytochemical observe of Olfactory ensheathing cells from human olfactory mucosa

作　　者：常瑞 梅强 阴小龙 程延 

机构地区：[1]西电集团医院创伤中心,陕西西安710077

出　　处：《生物骨科材料与临床研究》2008年第4期7-8,11,共3页Orthopaedic Biomechanics Materials and Clinical Study

基　　金：陕西省卫生厅基金资助项目(陕卫06252)

摘　　要：目的建立人鼻腔嗅粘膜嗅鞘细胞(OECs)的体外培养方法。方法利用鼻窦镜选取上鼻甲嗅区粘膜,原代培养嗅鞘细胞,进行形态学观察。结果成功培养出人鼻腔嗅粘膜OECs,为扁平的双极、多极形态,对P75、GFAP呈阳性反应,纯度在50%左右。结论人鼻腔嗅粘膜培养OECs的方法实用可行,但须在取材部位和纯化方面进一步探讨。Objective To establish the primary culture methods of Olfactory ensheathing cells from human olfactory mucosa. Methods OECs were acquired by Endoscopy from olfactory mucosa in superior turbinate, cultured and studied the morphological characteristics of OECs. Results OECs can be cultured from human olfactory mucosa, appeared long bipolar or unbipolar fusiform cell and identified with P75 and GFAP. The purity is 50%. Conclusion OECs can be acquired from human olfactory mucosa. The position of olfactory mucosa and purification required to investigation.

关 键 词：嗅粘膜 嗅鞘细胞 原代培养 

分 类 号：R765[医药卫生—耳鼻咽喉科]