大花蕙兰类原球茎增殖与分化影响因素的初步研究  

Preliminary Study on Factors Affecting in Multiplication and Differentiation of Protocorm Like-body of Cymbidium grandiflorum

作　　者：施隆文[1] 孟祥云[1] 庄应强[1] 费伟英[1] 毛军伟[1] 

机构地区：[1]嘉兴职业技术学院,浙江嘉兴314036

出　　处：《北方园艺》2008年第4期230-232,共3页Northern Horticulture

摘　　要：将大花蕙兰经扩繁5代后的类原球茎置于MS培养基中,以不同浓度的IBA、6-BA、活性炭进行正交试验,观察类原球茎的平均增殖倍数、分化率、平均芽生长数和平均根生长数。结果表明:IBA对类原球茎的平均增殖倍数、分化率、平均芽生长数的影响都极显著,6-BA对平均芽生长数的影响显著,活性炭对平均芽生长数的影响极显著。其较优的类原球茎增殖配方为:MS+IBA2.0mg/L+6-BA1.0mg/L+活性炭0.5g/L。The protocorm like-body（PLB） of Cymbidium granctiflorurn after multiplying 5 generations was put in MS medium,and factorial experiment with different level（concentration or content） of IBA,6-BA and active carbon was carried out,then results of average multiplicative times, different rate, average growth quantity of sprout and root of PLB were observed. After analysis, IBA affecting in average multiplicative times, different rate, average growth quantity of sprout of PLB was very significant, 6-BA affecting in average growth quantity of sprout of PLB was signiticant and active carbon affecting in average growth quantity of sprout was very significant. The dominant prescription for PLB＇s multiplication was as follows： MS＋ IBA 2.0 mg/L＋6-BA 1.0 mg/L＋ctive carbon 0.5 g/L.

关 键 词：大花蕙兰 类原球茎 增殖与分化 影响因素 

分 类 号：S682.29[农业科学—观赏园艺] S603.6[农业科学—园艺学]