蓝猪耳启动子捕获系统的建立  

Establishment of Promoter Trapping System in Torenia fournieri Linden

作　　者：龙海涛[1] 李洪清[1] 李玲[1] 

机构地区：[1]华南师范大学生命科学学院,广东广州510631

出　　处：《北方园艺》2008年第5期181-184,共4页Northern Horticulture

基　　金：广东省自然科学基金资助项目(003062)

摘　　要：为了分离基因启动子,构建了陷阱植物表达载体pHAHCA,用农杆菌介导的无启动子GUS方法转化蓝猪耳,经抗性筛选和PCR检测,获得82株转基因植株,转化率达12.5%。12株GUS染色呈阳性,其中10株GUS在叶脉表达,2株在茎和叶脉表达。对转基因植株进行盐胁迫处理,有1株根部出现GUS染色蓝色斑点。通过TAIL-PCR扩增和测序,获得5个T-DNA侧翼序列。Based on the optimization of genetic transformation of rorenia fournieri Linden mediated by Agrobacterium tumefaciens, a binary vector, designated as pHAHCA, was constructed for promoter trapping, and was introduced into Torenia to establish an efficient promoter trapping systerrL 82 hygromycin-resistant plants were confirmed to be positive transformants by PCR. The transformation frequency was up to 12. 5% overall. GUS histochemical staining of the 82 transgenic plants showed that 12 of them were stained. 10 out of the 12 transformants plants were stained only in veins of plants, and the other 2 transgenic plants were stained both in veins and stems. Under salt stress, only one plant showed GUS activity in roots. The flanking sequences of TDNA inserting sites in 5 transgenic Torenia showed GUS activity was amplified by TAIL-PCR.

关 键 词：启动子捕获 T-DNA侧翼 GUS报告基因 蓝猪耳 

分 类 号：S681.1[农业科学—观赏园艺]