柱前衍生高效液相色谱法测定白菜及小麦中草甘膦残留量  

HPLC Method for Determination of Glyphosate on Chinese Cabbage and Wheat

作　　者：马为民[1] 林小虎[1] 张燕[1] 张珊珊[1] 周印富[1] 

机构地区：[1]河北科技师范学院,河北秦皇岛066004

出　　处：《北方园艺》2008年第6期28-30,共3页Northern Horticulture

基　　金："十一五"国家科技支撑计划重大资助项目(2006BAB02A08-9)

摘　　要：运用荧光衍生试剂FMOC-Cl衍生草甘膦后,利用双聚合氨基柱HPLC测定了白菜及小麦中草甘膦的含量,该方法灵敏度高,可靠性强。通过试验,找到了最佳衍生条件和色谱条件。该方法通过测定蔬菜及小麦样品得到了确证,在添加草甘膦标样1mg/kg时,最低检出限为0.3mg/kg,平均回收率为82.4%,变异系数为10.3%。. This paper described a robust and sensitive HPLC method for determination of glyphosate on vegetable material after pre-derivatisation with 9-fluorenylmethyl chloroforrnate（FMOC-C1） using coupled polymeric amino columns. New findings about optimization of the derivatisation reaction and chromatographic behaviour. The method was validated using grass samples spiked at the level of 1 mg/kg and gave a detection limit of 0. 3 mg/kg for glyphosate and a recovery of 82.4% with RSD of 10.3%.

关 键 词：草甘膦 FMOC-Cl 高效液相色谱法 聚合氨基柱 柱前衍生 

分 类 号：Q94-331[生物学—植物学]